2asy: Difference between revisions

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[[Image:2asy.gif|left|200px]]
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[[Image:2asy.png|left|200px]]


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{{STRUCTURE_2asy|  PDB=2asy  |  SCENE=  }}  
{{STRUCTURE_2asy|  PDB=2asy  |  SCENE=  }}  


'''Solution Structure of ydhR protein from Escherichia coli'''
===Solution Structure of ydhR protein from Escherichia coli===




==Overview==
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YdhR is a 101-residue conserved protein from Escherichia coli. Sequence searches reveal that the protein has &gt;50% identity to proteins found in a variety of other bacterial genomes. Using size exclusion chromatography and fluorescence spectroscopy, we determined that ydhR exists in a dimeric state with a dissociation constant of approximately 40 nM. The three-dimensional structure of dimeric ydhR was determined using NMR spectroscopy. A total of 3400 unambiguous NOEs, both manually and automatically assigned, were used for the structure calculation that was refined using an explicit hydration shell. A family of 20 structures was obtained with a backbone RMSD of 0.48 A for elements of secondary structure. The structure reveals a dimeric alpha,beta fold characteristic of the alpha+beta barrel superfamily of proteins. Bioinformatic approaches were used to show that ydhR likely belongs to a recently identified group of mono-oxygenase proteins that includes ActVA-Orf6 and YgiN and are involved in the oxygenation of polyaromatic ring compounds.
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{{ABSTRACT_PUBMED_16260765}}


==About this Structure==
==About this Structure==
2ASY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ASY OCA].  
2ASY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ASY OCA].  


==Reference==
==Reference==
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[[Category: Ontario centre for structural proteomic]]
[[Category: Ontario centre for structural proteomic]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:26:28 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:43:02 2008''

Revision as of 23:43, 28 July 2008

File:2asy.png

Template:STRUCTURE 2asy

Solution Structure of ydhR protein from Escherichia coli

Template:ABSTRACT PUBMED 16260765

About this Structure

2ASY is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Solution structure of the Escherichia coli protein ydhR: a putative mono-oxygenase., Revington M, Semesi A, Yee A, Shaw GS, Protein Sci. 2005 Dec;14(12):3115-20. Epub 2005 Oct 31. PMID:16260765

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