1u7x: Difference between revisions

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[[Image:1u7x.gif|left|200px]]
{{Seed}}
[[Image:1u7x.png|left|200px]]


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{{STRUCTURE_1u7x|  PDB=1u7x  |  SCENE=  }}  
{{STRUCTURE_1u7x|  PDB=1u7x  |  SCENE=  }}  


'''crystal structure of a mutant M. jannashii tyrosyl-tRNA synthetase specific for O-methyl-tyrosine'''
===crystal structure of a mutant M. jannashii tyrosyl-tRNA synthetase specific for O-methyl-tyrosine===




==Overview==
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The Methanococcus jannaschii tRNA(Tyr)/TyrRS pair has been engineered to incorporate unnatural amino acids into proteins in E. coli. To reveal the structural basis for the altered specificity of mutant TyrRS for O-methyl-L-tyrosine (OMeTyr), the crystal structures for the apo wild-type and mutant M. jannaschii TyrRS were determined at 2.66 and 3.0 A, respectively, for comparison with the published structure of TyrRS complexed with tRNA(Tyr) and substrate tyrosine. A large conformational change was found for the anticodon recognition loop 257-263 of wild-type TyrRS upon tRNA binding in order to facilitate recognition of G34 of the anticodon loop through pi-stacking and hydrogen bonding interactions. Loop 133-143, which is close to the tRNA acceptor stem-binding site, also appears to be stabilized by interaction with the tRNA(Tyr). Binding of the substrate tyrosine results in subtle and cooperative movements of the side chains within the tyrosine-binding pocket. In the OMeTyr-specific mutant synthetase structure, the signature motif KMSKS loop and acceptor stem-binding loop 133-143 were surprisingly ordered in the absence of bound ATP and tRNA. The active-site mutations result in altered hydrogen bonding and steric interactions which favor binding of OMeTyr over L-tyrosine. The structure of the mutant and wild-type TyrRS now provide a basis for generating new active-site libraries to evolve synthetases specific for other unnatural amino acids.
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{{ABSTRACT_PUBMED_15840835}}


==About this Structure==
==About this Structure==
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[[Category: Zhang, Y.]]
[[Category: Zhang, Y.]]
[[Category: Rossman fold]]
[[Category: Rossman fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:52:14 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:50:27 2008''

Revision as of 23:50, 28 July 2008

File:1u7x.png

Template:STRUCTURE 1u7x

crystal structure of a mutant M. jannashii tyrosyl-tRNA synthetase specific for O-methyl-tyrosine

Template:ABSTRACT PUBMED 15840835

About this Structure

1U7X is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine., Zhang Y, Wang L, Schultz PG, Wilson IA, Protein Sci. 2005 May;14(5):1340-9. PMID:15840835

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