1qjf: Difference between revisions

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[[Image:1qjf.jpg|left|200px]]
{{Seed}}
[[Image:1qjf.png|left|200px]]


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{{STRUCTURE_1qjf|  PDB=1qjf  |  SCENE=  }}  
{{STRUCTURE_1qjf|  PDB=1qjf  |  SCENE=  }}  


'''ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (MONOCYCLIC SULFOXIDE-FE COMPLEX)'''
===ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (MONOCYCLIC SULFOXIDE-FE COMPLEX)===




==Overview==
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Isopenicillin N synthase (IPNS), a non-haem iron-dependent oxidase, catalyses the biosynthesis of isopenicillin N (IPN), the precursor of all penicillins and cephalosporins. The key steps in this reaction are the two iron-dioxygen-mediated ring closures of the tripeptide delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV). It has been proposed that the four-membered beta-lactam ring forms initially, associated with a highly oxidized iron(iv)-oxo (ferryl) moiety, which subsequently mediates closure of the five-membered thiazolidine ring. Here we describe observation of the IPNS reaction in crystals by X-ray crystallography. IPNS Fe2+ substrate crystals were grown anaerobically, exposed to high pressures of oxygen to promote reaction and frozen, and their structures were elucidated by X-ray diffraction. Using the natural substrate ACV, this resulted in the IPNS x Fe2+ x IPN product complex. With the substrate analogue, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-L-S-methylcysteine (ACmC) in the crystal, the reaction cycle was interrupted at the monocyclic stage. These mono- and bicyclic structures support our hypothesis of a two-stage reaction sequence leading to penicillin. Furthermore, the formation of a monocyclic sulphoxide product from ACmC is most simply explained by the interception of a high-valency iron-oxo species.
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{{ABSTRACT_PUBMED_10537113}}


==About this Structure==
==About this Structure==
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[[Category: Penicillin biosynthesis]]
[[Category: Penicillin biosynthesis]]
[[Category: Sulfoxide]]
[[Category: Sulfoxide]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:20:58 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:10:08 2008''

Revision as of 00:10, 29 July 2008

File:1qjf.png

Template:STRUCTURE 1qjf

ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (MONOCYCLIC SULFOXIDE-FE COMPLEX)

Template:ABSTRACT PUBMED 10537113

About this Structure

1QJF is a Single protein structure of sequence from Emericella nidulans. Full crystallographic information is available from OCA.

Reference

The reaction cycle of isopenicillin N synthase observed by X-ray diffraction., Burzlaff NI, Rutledge PJ, Clifton IJ, Hensgens CM, Pickford M, Adlington RM, Roach PL, Baldwin JE, Nature. 1999 Oct 14;401(6754):721-4. PMID:10537113

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