2o7c: Difference between revisions

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[[Image:2o7c.jpg|left|200px]]
{{Seed}}
[[Image:2o7c.png|left|200px]]


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{{STRUCTURE_2o7c|  PDB=2o7c  |  SCENE=  }}  
{{STRUCTURE_2o7c|  PDB=2o7c  |  SCENE=  }}  


'''Crystal structure of L-methionine-lyase from Pseudomonas'''
===Crystal structure of L-methionine-lyase from Pseudomonas===




==Overview==
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l-Methionine gamma-lyase (EC 4.4.1.11, MGL_Pp) from Pseudomonas putida is a multifunctional enzyme, which belongs to the gamma-family of pyridoxal-5'-phosphate (PLP) dependent enzymes. In this report, we demonstrate that the three-dimensional structure of MGL_Pp has been completely solved by the molecular replacement method to an R-factor of 20.4% at 1.8 A resolution. Detailed information of the overall structure of MGL_Pp supplies a clear picture of the substrate- and PLP-binding pockets. Tyr59 and Arg61 of neighbouring subunits, which are strongly conserved in other gamma-family enzymes, contact the phosphate group of PLP. These residues are important as the main anchor within the active site. Lys240, Asp241 and Arg61 of one partner monomer and Tyr114 and Cys116 of the other partner monomer form a hydrogen-bond network in the MGL active site which is specific for MGLs. It is also suggested that electrostatic interactions at the subunit interface are involved in the stabilization of the structural conformation. The detailed structure will facilitate the development of MGL_Pp as an anticancer drug.
The line below this paragraph, {{ABSTRACT_PUBMED_17289792}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_17289792}}


==About this Structure==
==About this Structure==
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[[Category: Methionine]]
[[Category: Methionine]]
[[Category: Plp]]
[[Category: Plp]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:24:47 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:18:20 2008''

Revision as of 00:18, 29 July 2008

File:2o7c.png

Template:STRUCTURE 2o7c

Crystal structure of L-methionine-lyase from Pseudomonas

Template:ABSTRACT PUBMED 17289792

About this Structure

2O7C is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Structure of the antitumour enzyme L-methionine gamma-lyase from Pseudomonas putida at 1.8 A resolution., Kudou D, Misaki S, Yamashita M, Tamura T, Takakura T, Yoshioka T, Yagi S, Hoffman RM, Takimoto A, Esaki N, Inagaki K, J Biochem. 2007 Apr;141(4):535-44. Epub 2007 Feb 8. PMID:17289792

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