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| {{STRUCTURE_2jdf| PDB=2jdf | SCENE= }} | | {{STRUCTURE_2jdf| PDB=2jdf | SCENE= }} |
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| '''HUMAN GAMMA-B CRYSTALLIN'''
| | ===HUMAN GAMMA-B CRYSTALLIN=== |
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| ==Overview==
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| The concept of novel binding proteins as an alternative to antibodies has undergone rapid development and is now ready for practical use in a wide range of applications. Alternative binding proteins, based on suitable scaffolds with desirable properties, are selected from combinatorial libraries in vitro. Here, we describe an approach using a beta-sheet of human gamma-B-crystallin to generate a universal binding site through randomization of eight solvent-exposed amino acid residues selected according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against a variety of targets that differ considerably in size and structure. The isolated Affilin variants can be produced in Escherichia coli as soluble proteins and have a high level of thermodynamic stability. The crystal structures of the human wild-type gamma-B-crystallin and a selected Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human gamma-B-crystallin tolerates amino acid exchanges with no major structural change. We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules. | | The line below this paragraph, {{ABSTRACT_PUBMED_17628592}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17628592 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17628592}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Polymorphism]] | | [[Category: Polymorphism]] |
| [[Category: Structural protein]] | | [[Category: Structural protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:44:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:34:30 2008'' |
