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| {{STRUCTURE_1vf6| PDB=1vf6 | SCENE= }} | | {{STRUCTURE_1vf6| PDB=1vf6 | SCENE= }} |
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| '''2.1 Angstrom crystal structure of the PALS-1-L27N and PATJ L27 heterodimer complex'''
| | ===2.1 Angstrom crystal structure of the PALS-1-L27N and PATJ L27 heterodimer complex=== |
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| ==Overview==
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| L27 is a protein-binding domain that can assemble essential proteins for signaling and cell polarity into complexes by interacting in a heterodimeric manner. One of these protein complexes is the PATJ/PALS1/Crumbs tripartite complex, which is crucial for the establishment and maintenance of cell polarity. To reveal the structural basis underlining the obligate heterodimerization, we have determined the crystal structure of the PALS1-L27N/PATJ-L27 heterodimer complex. Each L27 domain is composed of three helices. The two L27 domains heterodimerize by building a compact structure consisting of a four-helix bundle formed by the first two helices of each L27 domain and one coiled-coil formed by the third helix of each domain. The large hydrophobic packing interactions contributed by all the helices of both L27 domains predominantly drive the heterodimer formation, which is likely to be a general feature of L27 domains. Combined with mutational studies, we can begin to understand the structural basis for the specificity of L27 binding pairs. Our results provide unique insights into L27 domain heterodimer complex, which is critical for cell polarization.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15241471}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15241471 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15241471}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Hydrophobic packing interaction]] | | [[Category: Hydrophobic packing interaction]] |
| [[Category: L27 domain]] | | [[Category: L27 domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:28:16 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:36:57 2008'' |
