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| {{STRUCTURE_1y2a| PDB=1y2a | SCENE= }} | | {{STRUCTURE_1y2a| PDB=1y2a | SCENE= }} |
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| '''Structure of mammalian importin bound to the non-classical PLSCR1-NLS'''
| | ===Structure of mammalian importin bound to the non-classical PLSCR1-NLS=== |
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| ==Overview==
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| Nuclear import of proteins containing a classical nuclear localization signal (NLS) is an energy-dependent process that requires the heterodimer importin alpha/beta. Three to six basic contiguous arginine/lysine residues characterize a classical NLS and are thought to form a basic patch on the surface of the import cargo. In this study, we have characterized the NLS of phospholipid scramblase 1 (PLSCR1), a lipid-binding protein that enters the nucleus via the nonclassical NLS (257)GKISKHWTGI(266). This import sequence lacks a contiguous stretch of positively charged residues, and it is enriched in hydrophobic residues. We have determined the 2.2 A crystal structure of a complex between the PLSCR1 NLS and the armadillo repeat core of vertebrate importin alpha. Our crystallographic analysis reveals that PLSCR1 NLS binds to armadillo repeats 1-4 of importin alpha, but its interaction partially overlaps the classical NLS binding site. Two PLSCR1 lysines occupy the canonical positions indicated as P2 and P5. Moreover, we present in vivo evidence that the critical lysine at position P2, which is essential in other known NLS sequences, is dispensable in PLSCR1 NLS. Taken together, these data provide insight into a novel nuclear localization signal that presents a distinct motif for binding to importin alpha.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15611084}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15611084 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15611084}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein:peptide complex]] | | [[Category: Protein:peptide complex]] |
| [[Category: Superhelix of helice]] | | [[Category: Superhelix of helice]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:47:53 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:51:05 2008'' |
