1yka: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
Line 1: Line 1:
[[Image:1yka.gif|left|200px]]
{{Seed}}
[[Image:1yka.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1yka|  PDB=1yka  |  SCENE=  }}  
{{STRUCTURE_1yka|  PDB=1yka  |  SCENE=  }}  


'''Solution structure of Grx4, a monothiol glutaredoxin from E. coli.'''
===Solution structure of Grx4, a monothiol glutaredoxin from E. coli.===




==Overview==
<!--
The ubiquitous glutaredoxin protein family is present in both prokaryotes and eukaryotes, and is closely related to the thioredoxins, which reduce their substrates using a dithiol mechanism as part of the cellular defense against oxidative stress. Recently identified monothiol glutaredoxins, which must use a different functional mechanism, appear to be essential in both Escherichia coli and yeast and are well conserved in higher order genomes. We have employed high resolution NMR to determine the three-dimensional solution structure of a monothiol glutaredoxin, the reduced E. coli Grx4. The Grx4 structure comprises a glutaredoxin-like alpha-beta fold, founded on a limited set of strictly conserved and structurally critical residues. A tight hydrophobic core, together with a stringent set of secondary structure elements, is thus likely to be present in all monothiol glutaredoxins. A set of exposed and conserved residues form a surface region, implied in glutathione binding from a known structure of E. coli Grx3. The absence of glutaredoxin activity in E. coli Grx4 can be understood based on small but significant differences in the glutathione binding region, and through the lack of a conserved second GSH binding site. MALDI experiments suggest that disulfide formation on glutathionylation is accompanied by significant structural changes, in contrast with dithiol thioredoxins and glutaredoxins, where differences between oxidized and reduced forms are subtle and local. Structural and functional implications are discussed with particular emphasis on identifying common monothiol glutaredoxin properties in substrate specificity and ligand binding events, linking the thioredoxin and glutaredoxin systems.
The line below this paragraph, {{ABSTRACT_PUBMED_15840565}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15840565 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_15840565}}


==About this Structure==
==About this Structure==
1YKA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YKA OCA].  
1YKA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YKA OCA].  


==Reference==
==Reference==
Line 32: Line 36:
[[Category: Mixed alpha/beta fold]]
[[Category: Mixed alpha/beta fold]]
[[Category: Thioredoxin fold]]
[[Category: Thioredoxin fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:25:44 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:54:21 2008''

Revision as of 00:54, 29 July 2008

File:1yka.png

Template:STRUCTURE 1yka

Solution structure of Grx4, a monothiol glutaredoxin from E. coli.

Template:ABSTRACT PUBMED 15840565

About this Structure

1YKA is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Molecular mapping of functionalities in the solution structure of reduced Grx4, a monothiol glutaredoxin from Escherichia coli., Fladvad M, Bellanda M, Fernandes AP, Mammi S, Vlamis-Gardikas A, Holmgren A, Sunnerhagen M, J Biol Chem. 2005 Jul 1;280(26):24553-61. Epub 2005 Apr 18. PMID:15840565

Page seeded by OCA on Tue Jul 29 03:54:21 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1yka&oldid=725934"