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[[Image:2nnc.jpg|left|200px]]
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{{STRUCTURE_2nnc|  PDB=2nnc  |  SCENE=  }}  
{{STRUCTURE_2nnc|  PDB=2nnc  |  SCENE=  }}  


'''Structure of the sulfur carrier protein SoxY from Chlorobium limicola f thiosulfatophilum'''
===Structure of the sulfur carrier protein SoxY from Chlorobium limicola f thiosulfatophilum===




==Overview==
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Dissimilatory oxidation of thiosulfate in the green sulfur bacterium Chlorobium limicola f. thiosulfatophilum is carried out by the ubiquitous sulfur-oxidizing (Sox) multi-enzyme system. In this system, SoxY plays a key role, functioning as the sulfur substrate-binding protein that offers its sulfur substrate, which is covalently bound to a conserved C-terminal cysteine, to another oxidizing Sox enzyme. Here, we report the crystal structures of a stand-alone SoxY protein of C. limicola f. thiosulfatophilum, solved at 2.15 A and 2.40 A resolution using X-ray diffraction data collected at 100 K and room temperature, respectively. The structure reveals a monomeric Ig-like protein, with an N-terminal alpha-helix, that oligomerizes into a tetramer via conserved contact regions between the monomers. The tetramer can be described as a dimer of dimers that exhibits one large hydrophobic contact region in each dimer and two small hydrophilic interface patches in the tetramer. At the tetramer interface patch, two conserved redox-active C-terminal cysteines form an intersubunit disulfide bridge. Intriguingly, SoxY exhibits a dimer/tetramer equilibrium that is dependent on the redox state of the cysteines and on the type of sulfur substrate component bound to them. Taken together, the dimer/tetramer equilibrium, the specific interactions between the subunits in the tetramer, and the significant conservation level of the interfaces strongly indicate that these SoxY oligomers are biologically relevant.
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==About this Structure==
==About this Structure==
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[[Category: Soxy]]
[[Category: Soxy]]
[[Category: Sulfur binding protein]]
[[Category: Sulfur binding protein]]
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Revision as of 01:16, 29 July 2008

File:2nnc.png

Template:STRUCTURE 2nnc

Structure of the sulfur carrier protein SoxY from Chlorobium limicola f thiosulfatophilum

Template:ABSTRACT PUBMED 17327392

About this Structure

2NNC is a Single protein structure of sequence from Chlorobium limicola. Full crystallographic information is available from OCA.

Reference

X-ray crystallographic analysis of the sulfur carrier protein SoxY from Chlorobium limicola f. thiosulfatophilum reveals a tetrameric structure., Stout J, Van Driessche G, Savvides SN, Van Beeumen J, Protein Sci. 2007 Apr;16(4):589-601. Epub 2007 Feb 27. PMID:17327392

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