2b0h: Difference between revisions

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[[Image:2b0h.gif|left|200px]]
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[[Image:2b0h.png|left|200px]]


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{{STRUCTURE_2b0h|  PDB=2b0h  |  SCENE=  }}  
{{STRUCTURE_2b0h|  PDB=2b0h  |  SCENE=  }}  


'''Solution structure of VBS3 fragment of talin'''
===Solution structure of VBS3 fragment of talin===




==Overview==
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Talin is a key protein involved in linking integrins to the actin cytoskeleton. The long flexible talin rod domain contains a number of binding sites for vinculin, a cytoskeletal protein important in stabilizing integrin-mediated cell-matrix junctions. Here we report the solution structure of a talin rod polypeptide (residues 1843-1973) which contains a single vinculin binding site (VBS; residues 1944-1969). Like other talin rod polypeptides, it consists of a helical bundle, in this case a four-helix bundle with a right-handed topology. The residues in the VBS important for vinculin binding were identified by studying the binding of a series of VBS-related peptides to the vinculin Vd1 domain. The key binding determinants are buried in the interior of the helical bundle, suggesting that a substantial structural change in the talin polypeptide is required for vinculin binding. Direct evidence for this was obtained by NMR and EPR spectroscopy. [1H,15N]-HSQC spectra of the talin fragment indicate that vinculin binding caused approximately two-thirds of the protein to adopt a flexible random coil. For EPR spectroscopy, nitroxide spin labels were attached to the talin polypeptide via appropriately located cysteine residues. Measurements of inter-nitroxide distances in doubly spin-labeled protein showed clearly that the helical bundle is disrupted and the mobility of the helices, except for the VBS helix, is markedly increased. Binding of vinculin to talin is thus a clear example of the unusual phenomenon of protein unfolding being required for protein/protein interaction.
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{{ABSTRACT_PUBMED_16460027}}


==About this Structure==
==About this Structure==
2B0H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B0H OCA].  
2B0H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B0H OCA].  


==Reference==
==Reference==
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[[Category: Vb]]
[[Category: Vb]]
[[Category: Vinculin]]
[[Category: Vinculin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:42:04 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:28:30 2008''

Revision as of 01:28, 29 July 2008

File:2b0h.png

Template:STRUCTURE 2b0h

Solution structure of VBS3 fragment of talin

Template:ABSTRACT PUBMED 16460027

About this Structure

2B0H is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

Structural and dynamic characterization of a vinculin binding site in the talin rod., Gingras AR, Vogel KP, Steinhoff HJ, Ziegler WH, Patel B, Emsley J, Critchley DR, Roberts GC, Barsukov IL, Biochemistry. 2006 Feb 14;45(6):1805-17. PMID:16460027

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