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[[Image:2ici.gif|left|200px]]
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{{STRUCTURE_2ici|  PDB=2ici  |  SCENE=  }}  
{{STRUCTURE_2ici|  PDB=2ici  |  SCENE=  }}  


'''Crystal Structure of Streptococcal Pyrogenic Exotoxin I'''
===Crystal Structure of Streptococcal Pyrogenic Exotoxin I===




==Overview==
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Superantigens (SAgs) are potent microbial toxins that bind simultaneously to T cell receptors (TCRs) and class II major histocompatibility complex molecules, resulting in the activation and expansion of large T cell subsets and the onset of numerous human diseases. Within the bacterial SAg family, streptococcal pyrogenic exotoxin I (SpeI) has been classified as belonging to the group V SAg subclass, which are characterized by a unique, relatively conserved approximately 15 amino acid extension (amino acid residues 154 to 170 in SpeI; herein referred to as the alpha3-beta8 loop), absent in SAg groups I through IV. Here, we report the crystal structure of SpeI at 1.56 A resolution. Although the alpha3-beta8 loop in SpeI is several residues shorter than that of another group V SAg, staphylococcal enterotoxin serotype I, the C-terminal portions of these loops, which are located adjacent to the putative TCR binding site, are structurally similar. Mutagenesis and subsequent functional analysis of SpeI indicates that TCR beta-chains are likely engaged in a similar general orientation as other characterized SAgs. We show, however, that the alpha3-beta8 loop length, and the presence of key glycine residues, are necessary for optimal activation of T cells. Based on Vbeta-skewing analysis of human T cells activated with SpeI and structural models, we propose that the alpha3-beta8 loop is positioned to form productive intermolecular contacts with the TCR beta-chain, likely in framework region 3, and that these contacts are required for optimal TCR recognition by SpeI, and likely all other group V SAgs.
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{{ABSTRACT_PUBMED_17303163}}


==About this Structure==
==About this Structure==
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[[Category: Varma, A K.]]
[[Category: Varma, A K.]]
[[Category: Streptococcal superantigen spei]]
[[Category: Streptococcal superantigen spei]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:28:48 2008''

Revision as of 01:28, 29 July 2008

File:2ici.png

Template:STRUCTURE 2ici

Crystal Structure of Streptococcal Pyrogenic Exotoxin I

Template:ABSTRACT PUBMED 17303163

About this Structure

2ICI is a Single protein structure of sequence from Streptococcus pyogenes. Full crystallographic information is available from OCA.

Reference

Crystal structure of the streptococcal superantigen SpeI and functional role of a novel loop domain in T cell activation by group V superantigens., Brouillard JN, Gunther S, Varma AK, Gryski I, Herfst CA, Rahman AK, Leung DY, Schlievert PM, Madrenas J, Sundberg EJ, McCormick JK, J Mol Biol. 2007 Apr 6;367(4):925-34. Epub 2007 Jan 12. PMID:17303163

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