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| {{STRUCTURE_1vgj| PDB=1vgj | SCENE= }} | | {{STRUCTURE_1vgj| PDB=1vgj | SCENE= }} |
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| '''Crystal structure of 2'-5' RNA ligase from Pyrococcus horikoshii'''
| | ===Crystal structure of 2'-5' RNA ligase from Pyrococcus horikoshii=== |
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| ==Overview==
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| Bacterial and archaeal 2'-5' RNA ligases, members of the 2H phosphoesterase superfamily, catalyze the linkage of the 5' and 3' exons via a 2'-5'-phosphodiester bond during tRNA-precursor splicing. The crystal structure of the 2'-5' RNA ligase PH0099 from Pyrococcus horikoshii OT3 was solved at 1.94 A resolution (PDB code 1vgj). The molecule has a bilobal alpha+beta arrangement with two antiparallel beta-sheets constituting a V-shaped active-site cleft, as found in other members of the 2H phosphoesterase superfamily. The present structure was significantly different from that determined previously at 2.4 A resolution (PDB code 1vdx) in the active-site cleft; the entrance to the cleft is wider and the active site is easily accessible to the substrate (RNA precursor) in our structure. Structural comparison with the 2'-5' RNA ligase from Thermus thermophilus HB8 also revealed differences in the RNA precursor-binding region. The structural differences in the active-site residues (tetrapeptide motifs H-X-T/S-X) between the members of the 2H phosphoesterase superfamily are discussed.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17142895}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17142895 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17142895}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Ligt-like]] | | [[Category: Ligt-like]] |
| [[Category: Structural genomic]] | | [[Category: Structural genomic]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:30:58 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:49:15 2008'' |
