2c53: Difference between revisions

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[[Image:2c53.gif|left|200px]]
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{{STRUCTURE_2c53|  PDB=2c53  |  SCENE=  }}  
{{STRUCTURE_2c53|  PDB=2c53  |  SCENE=  }}  


'''A COMPARATIVE STUDY OF URACIL DNA GLYCOSYLASES FROM HUMAN AND HERPES SIMPLEX VIRUS TYPE 1'''
===A COMPARATIVE STUDY OF URACIL DNA GLYCOSYLASES FROM HUMAN AND HERPES SIMPLEX VIRUS TYPE 1===




==Overview==
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Uracil-DNA glycosylase (UNG) is the key enzyme responsible for initiation of base excision repair. We have used both kinetic and binding assays for comparative analysis of UNG enzymes from humans and herpes simplex virus type 1 (HSV-1). Steady-state fluorescence assays showed that hUNG has a much higher specificity constant (k(cat)/K(m)) compared with the viral enzyme due to a lower K(m). The binding of UNG to DNA was also studied using a catalytically inactive mutant of UNG and non-cleavable substrate analogs (2'-deoxypseudouridine and 2'-alpha-fluoro-2'-deoxyuridine). Equilibrium DNA binding revealed that both human and HSV-1 UNG enzymes bind to abasic DNA and both substrate analogs more weakly than to uracil-containing DNA. Structure determination of HSV-1 D88N/H210N UNG in complex with uracil revealed detailed information on substrate binding. Together, these results suggest that a significant proportion of the binding energy is provided by specific interactions with the target uracil. The kinetic parameters for human UNG indicate that it is likely to have activity against both U.A and U.G mismatches in vivo. Weak binding to abasic DNA also suggests that UNG activity is unlikely to be coupled to the subsequent common steps of base excision repair.
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{{ABSTRACT_PUBMED_16306042}}


==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Uracil dna glycosylase]]
[[Category: Uracil dna glycosylase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:15:47 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:14:56 2008''

Revision as of 02:15, 29 July 2008

File:2c53.png

Template:STRUCTURE 2c53

A COMPARATIVE STUDY OF URACIL DNA GLYCOSYLASES FROM HUMAN AND HERPES SIMPLEX VIRUS TYPE 1

Template:ABSTRACT PUBMED 16306042

About this Structure

2C53 is a Single protein structure of sequence from Human herpesvirus 1. Full crystallographic information is available from OCA.

Reference

A comparative study of uracil-DNA glycosylases from human and herpes simplex virus type 1., Krusong K, Carpenter EP, Bellamy SR, Savva R, Baldwin GS, J Biol Chem. 2006 Feb 24;281(8):4983-92. Epub 2005 Nov 22. PMID:16306042

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