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[[Image:2cqs.gif|left|200px]]
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[[Image:2cqs.png|left|200px]]


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{{STRUCTURE_2cqs|  PDB=2cqs  |  SCENE=  }}  
{{STRUCTURE_2cqs|  PDB=2cqs  |  SCENE=  }}  


'''Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate'''
===Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate===




==Overview==
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Cellobiose phosphorylase, a member of the glycoside hydrolase family 94, catalyses the reversible phosphorolysis of cellobiose into alpha-D-glucose 1-phosphate and D-glucose with inversion of the anomeric configuration. The substrate specificity and reaction mechanism of cellobiose phosphorylase from Cellvibrio gilvus have been investigated in detail. We have determined the crystal structure of the glucose-sulphate and glucose-phosphate complexes of this enzyme at a maximal resolution of 2.0 A (1 A=0.1 nm). The phosphate ion is strongly held through several hydrogen bonds, and the configuration appears to be suitable for direct nucleophilic attack to an anomeric centre. Structural features around the sugar-donor and sugar-acceptor sites were consistent with the results of extensive kinetic studies. When we compared this structure with that of homologous chitobiose phosphorylase, we identified key residues for substrate discrimination between glucose and N-acetylglucosamine in both the sugar-donor and sugar-acceptor sites. We found that the active site pocket of cellobiose phosphorylase was covered by an additional loop, indicating that some conformational change is required upon substrate binding. Information on the three-dimensional structure of cellobiose phosphorylase will facilitate engineering of this enzyme, the application of which to practical oligosaccharide synthesis has already been established.
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==About this Structure==
==About this Structure==
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[[Category: Wakagi, T.]]
[[Category: Wakagi, T.]]
[[Category: Beta-sandwich]]
[[Category: Beta-sandwich]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 22:50:03 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:18:07 2008''

Revision as of 02:18, 29 July 2008

File:2cqs.png

Template:STRUCTURE 2cqs

Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate

Template:ABSTRACT PUBMED 16646954

About this Structure

2CQS is a Single protein structure of sequence from Cellvibrio gilvus. Full crystallographic information is available from OCA.

Reference

Structural dissection of the reaction mechanism of cellobiose phosphorylase., Hidaka M, Kitaoka M, Hayashi K, Wakagi T, Shoun H, Fushinobu S, Biochem J. 2006 Aug 15;398(1):37-43. PMID:16646954

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