2res: Difference between revisions

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{{STRUCTURE_2res|  PDB=2res  |  SCENE=  }}  
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'''Tetracenomycin ARO/CYC mutant R69A'''
===Tetracenomycin ARO/CYC mutant R69A===




==Overview==
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Polyketides are a class of natural products with highly diverse chemical structures and pharmaceutical activities. Polyketide cyclization, promoted by the aromatase/cyclase (ARO/CYC), helps diversify aromatic polyketides. How the ARO/CYC promotes highly specific cyclization is not well understood because of the lack of a first-ring ARO/CYC structure. The 1.9 A crystal structure of Tcm ARO/CYC reveals that the enzyme belongs to the Bet v1-like superfamily (or STAR domain family) with a helix-grip fold, and contains a highly conserved interior pocket. Docking, mutagenesis, and an in vivo assay show that the size, shape, and composition of the pocket are important to orient and specifically fold the polyketide chain for C9-C14 first-ring and C7-C16 second-ring cyclizations. Two pocket residues, R69 and Y35, were found to be essential for promoting first- and second-ring cyclization specificity. Different pocket residue mutations affected the polyketide product distribution. A mechanism is proposed based on the structure-mutation-docking results. These results strongly suggest that the regiospecific cyclizations of the first two rings and subsequent aromatizations take place in the interior pocket. The chemical insights gleaned from this work pave the foundation toward defining the molecular rules for the ARO/CYC cyclization specificity, whose rational control will be important for future endeavors in the engineered biosynthesis of novel anticancer and antibiotic aromatic polyketides.
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{{ABSTRACT_PUBMED_18388203}}


==About this Structure==
==About this Structure==
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[[Category: Tetracenomycin]]
[[Category: Tetracenomycin]]
[[Category: Transferase]]
[[Category: Transferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:42:18 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:57:14 2008''

Revision as of 02:57, 29 July 2008

File:2res.png

Template:STRUCTURE 2res

Tetracenomycin ARO/CYC mutant R69A

Template:ABSTRACT PUBMED 18388203

About this Structure

2RES is a Single protein structure of sequence from Streptomyces glaucescens. Full crystallographic information is available from OCA.

Reference

Crystal structure and functional analysis of tetracenomycin ARO/CYC: implications for cyclization specificity of aromatic polyketides., Ames BD, Korman TP, Zhang W, Smith P, Vu T, Tang Y, Tsai SC, Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5349-54. Epub 2008 Apr 3. PMID:18388203

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