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| {{STRUCTURE_1o7l| PDB=1o7l | SCENE= }} | | {{STRUCTURE_1o7l| PDB=1o7l | SCENE= }} |
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| '''MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI'''
| | ===MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI=== |
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| ==Overview==
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| ModE is a bacterial transcriptional regulator that orchestrates many aspects of molybdenum metabolism by binding to specific DNA sequences in a molybdate-dependent fashion. We present the crystal structure of Escherichia coli ModE in complex with molybdate, which was determined at 2.75A from a merohedrally twinned crystal (twin fraction approximately 0.30) with space group P4(3). We now have structures of ModE in both its "switched on" (ligand-bound) and "switched off" (apo) states. Comparison with the apo structure shows that ligand binding leads to extensive conformational changes not only in the molybdate-binding domain, but also in the DNA-binding domain. The most obvious difference is the loss of the pronounced asymmetry between the two chains of the ModE dimer, which had been a characteristic property of the apo structure. Another major change concerns the relative orientation of the two DNA-interacting winged helix-turn-helix motifs. Manual docking of an idealized DNA structure suggests that this conformational change should improve DNA binding of the activated molybdate-bound ModE.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12581638}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12581638 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12581638}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Transcriptional regulator,activator]] | | [[Category: Transcriptional regulator,activator]] |
| [[Category: Winged helix-turn-helix]] | | [[Category: Winged helix-turn-helix]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:29:08 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:24:19 2008'' |
