2gtj: Difference between revisions

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[[Image:2gtj.jpg|left|200px]]
{{Seed}}
[[Image:2gtj.png|left|200px]]


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{{STRUCTURE_2gtj|  PDB=2gtj  |  SCENE=  }}  
{{STRUCTURE_2gtj|  PDB=2gtj  |  SCENE=  }}  


'''Reduced form of ADAP hSH3-N-domain'''
===Reduced form of ADAP hSH3-N-domain===




==Overview==
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Oxidation-induced conformational changes in proteins provide a powerful mechanism to sense the redox state of a living cell. In contrast to the unspecific and often irreversible oxidation of intracellular proteins during severe oxidative stress, regulatory redox events need to have specific and transient effects on cellular targets. Here we present evidence for the reversible formation of a vicinal disulfide bond in a prototypic protein interaction domain. NMR spectroscopy was used to determine the structure of the N-terminal hSH3 domain (hSH3N) of the immune cell protein ADAP (adhesion and degranulation promoting adapter protein) in the reduced and oxidized states. An eight-membered ring formed upon oxidation of two neighboring cysteines leads to significant changes in the variable arginine-threonine (RT) loop of the hSH3N domain and alters the helix-sheet packing of the domain. The redox potential for this structural transition is -228 mV at pH 7.4. This is compatible with a role of the cysteinylcysteine moiety in redox signaling during T cell activation.
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{{ABSTRACT_PUBMED_17511475}}


==About this Structure==
==About this Structure==
2GTJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTJ OCA].  
2GTJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTJ OCA].  


==Reference==
==Reference==
Redox-regulated conformational changes in an SH3 domain., Zimmermann J, Kuhne R, Sylvester M, Freund C, Biochemistry. 2007 Jun 12;46(23):6971-7. Epub 2007 May 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17511475 17511475]
Redox-regulated conformational changes in an SH3 domain., Zimmermann J, Kuhne R, Sylvester M, Freund C, Biochemistry. 2007 Jun 12;46(23):6971-7. Epub 2007 May 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17511475 17511475]
NMR assignment of the reduced and oxidized forms of the human ADAP hSH3-1 domain., Zimmermann J, Freund C, J Biomol NMR. 2005 May;32(1):94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16041492 16041492]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Redox]]
[[Category: Redox]]
[[Category: Sh3]]
[[Category: Sh3]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:31:05 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:26:31 2008''

Revision as of 03:26, 29 July 2008

File:2gtj.png

Template:STRUCTURE 2gtj

Reduced form of ADAP hSH3-N-domain

Template:ABSTRACT PUBMED 17511475

About this Structure

2GTJ is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Redox-regulated conformational changes in an SH3 domain., Zimmermann J, Kuhne R, Sylvester M, Freund C, Biochemistry. 2007 Jun 12;46(23):6971-7. Epub 2007 May 19. PMID:17511475

NMR assignment of the reduced and oxidized forms of the human ADAP hSH3-1 domain., Zimmermann J, Freund C, J Biomol NMR. 2005 May;32(1):94. PMID:16041492

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