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| {{STRUCTURE_1slq| PDB=1slq | SCENE= }} | | {{STRUCTURE_1slq| PDB=1slq | SCENE= }} |
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| '''Crystal structure of the trimeric state of the rhesus rotavirus VP4 membrane interaction domain, VP5CT'''
| | ===Crystal structure of the trimeric state of the rhesus rotavirus VP4 membrane interaction domain, VP5CT=== |
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| ==Overview==
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| Non-enveloped virus particles (those that lack a lipid-bilayer membrane) must breach the membrane of a target host cell to gain access to its cytoplasm. So far, the molecular mechanism of this membrane penetration step has resisted structural analysis. The spike protein VP4 is a principal component in the entry apparatus of rotavirus, a non-enveloped virus that causes gastroenteritis and kills 440,000 children each year. Trypsin cleavage of VP4 primes the virus for entry by triggering a rearrangement that rigidifies the VP4 spikes. We have determined the crystal structure, at 3.2 A resolution, of the main part of VP4 that projects from the virion. The crystal structure reveals a coiled-coil stabilized trimer. Comparison of this structure with the two-fold clustered VP4 spikes in a approximately 12 A resolution image reconstruction from electron cryomicroscopy of trypsin-primed virions shows that VP4 also undergoes a second rearrangement, in which the oligomer reorganizes and each subunit folds back on itself, translocating a potential membrane-interaction peptide from one end of the spike to the other. This rearrangement resembles the conformational transitions of membrane fusion proteins of enveloped viruses.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15329727}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15329727 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15329727}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Non-enveloped virus]] | | [[Category: Non-enveloped virus]] |
| [[Category: Spike protein]] | | [[Category: Spike protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:51:37 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:22:45 2008'' |
