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| {{STRUCTURE_1mtx| PDB=1mtx | SCENE= }} | | {{STRUCTURE_1mtx| PDB=1mtx | SCENE= }} |
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| '''DETERMINATION OF THE THREE-DIMENSIONAL STRUCTURE OF MARGATOXIN BY 1H, 13C, 15N TRIPLE-RESONANCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY'''
| | ===DETERMINATION OF THE THREE-DIMENSIONAL STRUCTURE OF MARGATOXIN BY 1H, 13C, 15N TRIPLE-RESONANCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY=== |
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| ==Overview==
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| The solution structure of the 39-residue peptide margatoxin, a scorpion toxin that selectively blocks the voltage-gated potassium-channel Kv1.3, has been determined by NMR spectroscopy. The toxin was isotopically labeled with 13C and 15N and studied using two-dimensional homonuclear and three- and four-dimensional heteronuclear NMR spectroscopy. The final structure was determined using 501 constraints, comprising 422 NOE constraints, 60 dihedral angle constraints, 9 disulfide constraints, and 10 hydrogen bond constraints. Structures were initially determined with the program PEGASUS and subsequently refined with X-PLOR. The average rms deviation from a calculated average structure for the backbone atoms of residues 3-38 is 0.40 A. A helix is present from residues 11 to 20 and includes two proline residues at positions 15 and 16. A loop at residues 21-24 leads into a two-strand antiparallel sheet from residues 25 to 38 with a turn at residues 30-33. Residues 3-6 run adjacent to the 33-38 strand but do not form a canonical beta-strand. The two additional residues of margatoxin, relative to the related toxins charybdotoxin and iberiotoxin, insert in a manner that extends the beta-sheet by one residue. Otherwise, the global structure is very similar to that of these two other toxins. The longer sheet may have implications for channel selectivity.
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| | (as it appears on PubMed at http://www.pubmed.gov), where 7999764 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_7999764}} |
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| ==About this Structure== | | ==About this Structure== |
| 1MTX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_margaritatus Centruroides margaritatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTX OCA]. | | 1MTX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_margaritatus Centruroides margaritatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTX OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Williamson, J M.]] | | [[Category: Williamson, J M.]] |
| [[Category: Toxin]] | | [[Category: Toxin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:42:39 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:09:21 2008'' |
