2i1v: Difference between revisions

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[[Image:2i1v.jpg|left|200px]]
{{Seed}}
[[Image:2i1v.png|left|200px]]


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{{STRUCTURE_2i1v|  PDB=2i1v  |  SCENE=  }}  
{{STRUCTURE_2i1v|  PDB=2i1v  |  SCENE=  }}  


'''Crystal structure of PFKFB3 in complex with ADP and Fructose-2,6-bisphosphate'''
===Crystal structure of PFKFB3 in complex with ADP and Fructose-2,6-bisphosphate===




==Overview==
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To understand the molecular basis of a phosphoryl transfer reaction catalyzed by the 6-phosphofructo-2-kinase domain of the hypoxia-inducible bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3), the crystal structures of PFKFB3AMPPCPfructose-6-phosphate and PFKFB3ADPphosphoenolpyruvate complexes were determined to 2.7 A and 2.25 A resolution, respectively. Kinetic studies on the wild-type and site-directed mutant proteins were carried out to confirm the structural observations. The experimentally varied liganding states in the active pocket cause no significant conformational changes. In the pseudo-substrate complex, a strong direct interaction between AMPPCP and fructose-6-phosphate (Fru-6-P) is found. By virtue of this direct substrate-substrate interaction, Fru-6-P is aligned with AMPPCP in an orientation and proximity most suitable for a direct transfer of the gamma-phosphate moiety to 2-OH of Fru-6-P. The three key atoms involved in the phosphoryl transfer, the beta,gamma-phosphate bridge oxygen atom, the gamma-phosphorus atom, and the 2-OH group are positioned in a single line, suggesting a direct phosphoryl transfer without formation of a phosphoenzyme intermediate. In addition, the distance between 2-OH and gamma-phosphorus allows the gamma-phosphate oxygen atoms to serve as a general base catalyst to induce an "associative" phosphoryl transfer mechanism. The site-directed mutant study and inhibition kinetics suggest that this reaction will be catalyzed most efficiently by the protein when the substrates bind to the active pocket in an ordered manner in which ATP binds first.
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{{ABSTRACT_PUBMED_17499765}}


==About this Structure==
==About this Structure==
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[[Category: Ternary product complex]]
[[Category: Ternary product complex]]
[[Category: Transferase]]
[[Category: Transferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May  7 08:44:20 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:19:37 2008''

Revision as of 05:19, 29 July 2008

File:2i1v.png

Template:STRUCTURE 2i1v

Crystal structure of PFKFB3 in complex with ADP and Fructose-2,6-bisphosphate

Template:ABSTRACT PUBMED 17499765

About this Structure

2I1V is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase., Kim SG, Cavalier M, El-Maghrabi MR, Lee YH, J Mol Biol. 2007 Jun 29;370(1):14-26. Epub 2007 Mar 21. PMID:17499765

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