3bpb: Difference between revisions

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[[Image:3bpb.jpg|left|200px]]
{{Seed}}
[[Image:3bpb.png|left|200px]]


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{{STRUCTURE_3bpb|  PDB=3bpb  |  SCENE=  }}  
{{STRUCTURE_3bpb|  PDB=3bpb  |  SCENE=  }}  


'''Crystal structure of the dimethylarginine dimethylaminohydrolase H162G adduct with S-methyl-L-thiocitrulline'''
===Crystal structure of the dimethylarginine dimethylaminohydrolase H162G adduct with S-methyl-L-thiocitrulline===




==Overview==
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Many enzymes in the pentein superfamily use a transient covalent intermediate in their catalytic mechanisms. Here we trap and determine the structure of a stable covalent adduct that mimics this intermediate using a mutant dimethylarginine dimethylaminohydrolase and an alternative substrate. The interactions observed between the enzyme and trapped adduct suggest an altered angle of attack between the nucleophiles of the first and second half-reactions of normal catalysis. The stable covalent adduct is also capable of further reaction. Addition of imidazole rescues the original hydrolytic activity. Notably, addition of other amines instead yields substituted arginine products, which arise from partitioning of the intermediate into the evolutionarily related amidinotransferase reaction pathway. The enzyme provides both selectivity and catalysis for the amidinotransferase reaction, underscoring commonalities among the reaction pathways in this mechanistically diverse enzyme superfamily. The promiscuous partitioning of this intermediate may also help to illuminate the evolutionary history of these enzymes.
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==About this Structure==
==About this Structure==
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[[Category: Enzyme adduct]]
[[Category: Enzyme adduct]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:08:16 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:23:42 2008''

Revision as of 05:23, 29 July 2008

File:3bpb.png

Template:STRUCTURE 3bpb

Crystal structure of the dimethylarginine dimethylaminohydrolase H162G adduct with S-methyl-L-thiocitrulline

Template:ABSTRACT PUBMED 18482699

About this Structure

3BPB is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Promiscuous partitioning of a covalent intermediate common in the pentein superfamily., Linsky TW, Monzingo AF, Stone EM, Robertus JD, Fast W, Chem Biol. 2008 May;15(5):467-75. PMID:18482699

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