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| [[Image:1nbp.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1nbp| PDB=1nbp | SCENE= }} | | {{STRUCTURE_1nbp| PDB=1nbp | SCENE= }} |
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| '''Crystal Structure Of Human Interleukin-2 Y31C Covalently Modified At C31 With 3-Mercapto-1-(1,3,4,9-tetrahydro-B-carbolin-2-yl)-propan-1-one'''
| | ===Crystal Structure Of Human Interleukin-2 Y31C Covalently Modified At C31 With 3-Mercapto-1-(1,3,4,9-tetrahydro-B-carbolin-2-yl)-propan-1-one=== |
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| ==Overview==
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| The cytokine hormone interleukin-2 (IL-2) contains a highly adaptive region that binds small, druglike molecules. The binding properties of this adaptive region have been explored using a "tethering" method that relies on the formation of a disulfide bond between the protein and small-molecule ligands. Using tethering, surface plasmon resonance (SPR), and X-ray crystallography, we have discovered that the IL-2 adaptive region contains at least two cooperative binding sites where the binding of a first ligand to one site promotes or antagonizes the binding of a second ligand to the second site. Cooperative energies of interaction of -2 kcal/mol are observed. The observation that the adaptive region contains two adjacent sites may lead to the development of tight-binding antagonists of a protein-protein interaction. Cooperative ligand binding in the adaptive region of IL-2 underscores the importance of protein dynamics in molecular recognition. The tethering approach provides a novel and general strategy for discovering such cooperative binding interactions in specific, flexible regions of protein structure.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12767230}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12767230 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12767230}} |
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| ==Disease== | | ==Disease== |
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| [[Category: Four-helix bundle]] | | [[Category: Four-helix bundle]] |
| [[Category: Small molecule complex]] | | [[Category: Small molecule complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:20:22 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:27:28 2008'' |
