2fqf: Difference between revisions

Line 1:

[[Image:2fqf.~~jpg~~|left|200px]]

[[Image:2fqf.png|left|200px]]

<!--

Line 9:

Line 10:

~~'''~~Crystal Structures of E. coli Laccase CueO under different copper binding situations~~'''~~

===Crystal Structures of E. coli Laccase CueO under different copper binding situations===

~~==Overview==~~

<!--

~~CueO protein is a hypothetical bacterial laccase and a good laccase candidate for large scale industrial application. Four CueO crystal structures were determined~~ at ~~different copper concentrations~~. ~~Low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper were demonstrated~~. ~~These observations well explain~~ the ~~copper dependence of CueO oxidase activity~~. ~~Structural comparison between CueO and other three fungal laccase proteins indicates that Glu106 in CueO constitutes the primary counter~~-~~work for reconstitution of the trinuclear copper site. Mutation of Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis. In addition, an extra alpha~~-~~helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper.~~

The line below this paragraph, {{ABSTRACT_PUBMED_17217912}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17217912 is the PubMed ID number.

-->

==About this Structure==

Line 28:

Line 32:

[[Category: Zhang, M.]]

[[Category: Azurin-like domain]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 04~~:12:06 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:47:43 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:2fqf.jpg|left|200px]]
+{{Seed}}
+[[Image:2fqf.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_2fqf|  PDB=2fqf  |  SCENE=  }}
-'''Crystal Structures of E. coli Laccase CueO under different copper binding situations'''
+===Crystal Structures of E. coli Laccase CueO under different copper binding situations===
-==Overview==
+<!--
-CueO protein is a hypothetical bacterial laccase and a good laccase candidate for large scale industrial application. Four CueO crystal structures were determined at different copper concentrations. Low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper were demonstrated. These observations well explain the copper dependence of CueO oxidase activity. Structural comparison between CueO and other three fungal laccase proteins indicates that Glu106 in CueO constitutes the primary counter-work for reconstitution of the trinuclear copper site. Mutation of Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis. In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper.
+The line below this paragraph, {{ABSTRACT_PUBMED_17217912}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17217912 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17217912}}
 ==About this Structure==
@@ Line 28: / Line 32: @@
 [[Category: Zhang, M.]]
 [[Category: Azurin-like domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:12:06 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:47:43 2008''