2ale: Difference between revisions

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[[Image:2ale.jpg|left|200px]]
{{Seed}}
[[Image:2ale.png|left|200px]]


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{{STRUCTURE_2ale|  PDB=2ale  |  SCENE=  }}  
{{STRUCTURE_2ale|  PDB=2ale  |  SCENE=  }}  


'''Crystal structure of yeast RNA splicing factor Snu13p'''
===Crystal structure of yeast RNA splicing factor Snu13p===




==Overview==
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Snu13p is a Saccharomyces cerevisiae protein essential for pre-messenger RNA splicing and pre-ribosomal RNA processing. Snu13p binds U4 snRNA of the spliceosome and box C/D snoRNAs of the pre-ribosomal RNA processing machinery to induce assembly of each ribonucleoprotein complex. Here, we present structural and biochemical analysis of Snu13p. The crystal structure of Snu13p reveals a region of the protein which could be important for protein interaction during ribonucleoprotein assembly. Using the structure of Snu13p we have designed the first temperature-sensitive mutants in Snu13p, L67W and I102A. Wild-type and mutant Snu13p proteins were assayed for binding to U4 snRNA and U3 snoRNA. Both temperature-sensitive mutants displayed significantly reduced RNA binding compared to wild-type protein. As the temperature-sensitive mutations are not in the known RNA binding region of Snu13p this indicates that these mutants indirectly influence the RNA binding properties of Snu13p. This work provides insight into Snu13p function during ribonucleoprotein assembly.
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{{ABSTRACT_PUBMED_17631273}}


==About this Structure==
==About this Structure==
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[[Category: Splicing]]
[[Category: Splicing]]
[[Category: Yeast]]
[[Category: Yeast]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:11:18 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:02:29 2008''

Revision as of 06:02, 29 July 2008

File:2ale.png

Template:STRUCTURE 2ale

Crystal structure of yeast RNA splicing factor Snu13p

Template:ABSTRACT PUBMED 17631273

About this Structure

2ALE is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Analysis of pre-mRNA and pre-rRNA processing factor Snu13p structure and mutants., Dobbyn HC, McEwan PA, Krause A, Novak-Frazer L, Bella J, O'Keefe RT, Biochem Biophys Res Commun. 2007 Sep 7;360(4):857-62. Epub 2007 Jul 9. PMID:17631273

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