2bk9: Difference between revisions

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[[Image:2bk9.gif|left|200px]]
{{Seed}}
[[Image:2bk9.png|left|200px]]


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{{STRUCTURE_2bk9|  PDB=2bk9  |  SCENE=  }}  
{{STRUCTURE_2bk9|  PDB=2bk9  |  SCENE=  }}  


'''DROSOPHILA MELANOGASTER GLOBIN'''
===DROSOPHILA MELANOGASTER GLOBIN===




==Overview==
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Hemoglobins at high concentration have been isolated long ago from some insect larvae living in hypoxic environments. Conversely, a monomeric hemoglobin has been discovered recently in the fruit fly Drosophila melanogaster as intracellular protein expressed both in larvae and in the adult fly. Such a finding indicates that the oxygen supply in insects may be more complex than previously thought, relying not only on O2 diffusion through the tubular tracheal system, but also on carrier-mediated transport and storage. We present here the crystal structure of recombinant D. melanogaster hemoglobin at 1.20 A resolution. Spectroscopic data show that the protein displays a hexacoordinated heme, whose axial ligands are the proximal and distal His residues. Such bis-His ligation of the heme has sizable effects on the protein local structure. Three protein matrix cavities, comparable in size but not in topological locations with those of sperm whale myoglobin, are spread through the protein matrix; one of these can host a xenon atom. Additionally, D. melanogaster hemoglobin binds one molecule of 3-(cyclohexylamino)propanesulfonic acid (CAPS) buffer at a surface pocket, next to the EF hinge. Despite the high resolution achieved, no sequence/structure features specifically supporting the heme hexa- to pentacoordination transition required for diatomic ligand binding could be recognized.
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{{ABSTRACT_PUBMED_15917230}}


==About this Structure==
==About this Structure==
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[[Category: Protein cavity]]
[[Category: Protein cavity]]
[[Category: Protein structure oxygen transport]]
[[Category: Protein structure oxygen transport]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:24:15 2008''
 
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Revision as of 06:05, 29 July 2008

File:2bk9.png

Template:STRUCTURE 2bk9

DROSOPHILA MELANOGASTER GLOBIN

Template:ABSTRACT PUBMED 15917230

About this Structure

2BK9 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Bishistidyl heme hexacoordination, a key structural property in Drosophila melanogaster hemoglobin., de Sanctis D, Dewilde S, Vonrhein C, Pesce A, Moens L, Ascenzi P, Hankeln T, Burmester T, Ponassi M, Nardini M, Bolognesi M, J Biol Chem. 2005 Jul 22;280(29):27222-9. Epub 2005 May 24. PMID:15917230

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