1mwn: Difference between revisions

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[[Image:1mwn.jpg|left|200px]]
{{Seed}}
[[Image:1mwn.png|left|200px]]


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{{STRUCTURE_1mwn|  PDB=1mwn  |  SCENE=  }}  
{{STRUCTURE_1mwn|  PDB=1mwn  |  SCENE=  }}  


'''Solution NMR structure of S100B bound to the high-affinity target peptide TRTK-12'''
===Solution NMR structure of S100B bound to the high-affinity target peptide TRTK-12===




==Overview==
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The solution NMR structure is reported for Ca(2+)-loaded S100B bound to a 12-residue peptide, TRTK-12, from the actin capping protein CapZ (alpha1 or alpha2 subunit, residues 265-276: TRTKIDWNKILS). This peptide was discovered by Dimlich and co-workers by screening a bacteriophage random peptide display library, and it matches exactly the consensus S100B binding sequence ((K/R)(L/I)XWXXIL). As with other S100B target proteins, a calcium-dependent conformational change in S100B is required for TRTK-12 binding. The TRTK-12 peptide is an amphipathic helix (residues W7 to S12) in the S100B-TRTK complex, and helix 4 of S100B is extended by three or four residues upon peptide binding. However, helical TRTK-12 in the S100B-peptide complex is uniquely oriented when compared to the three-dimensional structures of other S100-peptide complexes. The three-dimensional structure of the S100B-TRTK peptide complex illustrates that residues in the S100B binding consensus sequence (K4, I5, W7, I10, L11) are all involved in the S100B-peptide interface, which can explain its orientation in the S100B binding pocket and its relatively high binding affinity. A comparison of the S100B-TRTK peptide structure to the structures of apo- and Ca(2+)-bound S100B illustrates that the binding site of TRTK-12 is buried in apo-S100B, but is exposed in Ca(2+)-bound S100B as necessary to bind the TRTK-12 peptide.
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{{ABSTRACT_PUBMED_12470955}}


==About this Structure==
==About this Structure==
1MWN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWN OCA].  
1MWN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWN OCA].  


==Reference==
==Reference==
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[[Category: S100b]]
[[Category: S100b]]
[[Category: Trtk-12]]
[[Category: Trtk-12]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:48:37 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:21:38 2008''

Revision as of 06:21, 29 July 2008

File:1mwn.png

Template:STRUCTURE 1mwn

Solution NMR structure of S100B bound to the high-affinity target peptide TRTK-12

Template:ABSTRACT PUBMED 12470955

About this Structure

1MWN is a Protein complex structure of sequences from Rattus norvegicus. Full experimental information is available from OCA.

Reference

Solution NMR structure of S100B bound to the high-affinity target peptide TRTK-12., Inman KG, Yang R, Rustandi RR, Miller KE, Baldisseri DM, Weber DJ, J Mol Biol. 2002 Dec 13;324(5):1003-14. PMID:12470955

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