1u9l: Difference between revisions

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[[Image:1u9l.gif|left|200px]]
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[[Image:1u9l.png|left|200px]]


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{{STRUCTURE_1u9l|  PDB=1u9l  |  SCENE=  }}  
{{STRUCTURE_1u9l|  PDB=1u9l  |  SCENE=  }}  


'''Structural basis for a NusA- protein N interaction'''
===Structural basis for a NusA- protein N interaction===




==Overview==
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The C terminus of transcription factor NusA from Escherichia coli comprises two repeat units, which bind during antitermination to protein N from phage lambda. To delineate the structural basis of the NusA-lambdaN interaction, we attempted to crystallize the NusA C-terminal repeats in complex with a lambdaN peptide (residues 34-47). The two NusA domains became proteolytically separated during crystallization, and crystals contained two copies of the first repeat unit in contact with a single lambdaN fragment. The NusA modules employ identical regions to contact the peptide but approach the ligand from opposite sides. In contrast to the alpha-helical conformation of the lambdaN N terminus in complex with boxB RNA, residues 34-40 of lambdaN remain extended upon interaction with NusA. Mutational analyses indicated that only one of the observed NusA-lambdaN interaction modes is biologically significant, supporting an equimolar ratio of NusA and lambdaN in antitermination complexes. Solution studies indicated that additional interactions are fostered by the second NusA repeat unit, consistent with known compensatory mutations in NusA and lambdaN. Contrary to the RNA polymerase alpha subunit, lambdaN binding does not stimulate RNA interaction of NusA. The results demonstrate that lambdaN serves as a scaffold to closely oppose NusA and the mRNA in antitermination complexes.
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{{ABSTRACT_PUBMED_15365170}}


==About this Structure==
==About this Structure==
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[[Category: Transcription antitermination]]
[[Category: Transcription antitermination]]
[[Category: X-ray crystallography]]
[[Category: X-ray crystallography]]
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Revision as of 07:06, 29 July 2008

File:1u9l.png

Template:STRUCTURE 1u9l

Structural basis for a NusA- protein N interaction

Template:ABSTRACT PUBMED 15365170

About this Structure

1U9L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural basis for the interaction of Escherichia coli NusA with protein N of phage lambda., Bonin I, Muhlberger R, Bourenkov GP, Huber R, Bacher A, Richter G, Wahl MC, Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13762-7. Epub 2004 Sep 13. PMID:15365170

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