2hf9: Difference between revisions

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[[Image:2hf9.gif|left|200px]]
{{Seed}}
[[Image:2hf9.png|left|200px]]


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{{STRUCTURE_2hf9|  PDB=2hf9  |  SCENE=  }}  
{{STRUCTURE_2hf9|  PDB=2hf9  |  SCENE=  }}  


'''Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form'''
===Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form===




==Overview==
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HypB is a prokaryotic metal-binding guanine nucleotide-binding protein that is essential for nickel incorporation into hydrogenases. Here we solved the x-ray structure of HypB from Methanocaldococcus jannaschii. It shows that the G-domain has a different topology than the Ras-like proteins and belongs to the SIMIBI (after Signal Recognition Particle, MinD and BioD) class of NTP-binding proteins. We show that HypB undergoes nucleotide-dependent dimerization, which is apparently a common feature of SIMIBI class G-proteins. The nucleotides are located in the dimer interface and are contacted by both subunits. The active site features residues from both subunits arguing that hydrolysis also requires dimerization. Two metal-binding sites are found, one of which is dependent on the state of bound nucleotide. A totally conserved ENV/IGNLV/ICP motif in switch II relays the nucleotide binding with the metal ionbinding site. The homology with NifH, the Fe protein subunit of nitrogenase, suggests a mechanistic model for the switch-dependent incorporation of a metal ion into hydrogenases.
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{{ABSTRACT_PUBMED_16807243}}


==About this Structure==
==About this Structure==
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[[Category: Alpha and beta protein]]
[[Category: Alpha and beta protein]]
[[Category: P-loop containing nucleoside triphosphate hydrolase]]
[[Category: P-loop containing nucleoside triphosphate hydrolase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:13:24 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:42:39 2008''

Revision as of 07:42, 29 July 2008

File:2hf9.png

Template:STRUCTURE 2hf9

Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form

Template:ABSTRACT PUBMED 16807243

About this Structure

2HF9 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

Structural insights into HypB, a GTP-binding protein that regulates metal binding., Gasper R, Scrima A, Wittinghofer A, J Biol Chem. 2006 Sep 15;281(37):27492-502. Epub 2006 Jun 28. PMID:16807243

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