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| {{STRUCTURE_2avf| PDB=2avf | SCENE= }} | | {{STRUCTURE_2avf| PDB=2avf | SCENE= }} |
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| '''Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes'''
| | ===Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes=== |
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| ==Overview==
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| Monoclinic crystal structure of C-terminal desundecapeptide nitrite reductase (NiRc-11) from Achromobacter cycloclastes was determined at 2.6A. NiRc-11 exists as a loose trimer in the crystal. Deletion of 11 residues eliminates all intersubunit hydrogen bonds mediated by the C-terminal tail. The rigid irregular coil 105-112, which constitutes part of the sidewall of the active site pocket, undergoes conformational changes and becomes highly flexible in NiRc-11. Correspondingly, the linker segments between the two copper sites 95-100 and 135-136 are partly relaxed in conformation, which leads to disrupted active site microenvironments responsible for the activity loss and spectral change of NiRc-11. Comparison with the native structure revealed a bulky residue Met331 fastened by hydrogen bonding, which may play a direct role in keeping the right copper site geometry by protruding its side chain against the irregular coil 105-112. Sequence alignment showed that the bulky residue is conserved at position 331, indicating an equal importance of C-terminal segment in other copper-containing nitrite reductases.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16293231}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16293231 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16293231}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Zhang, J P.]] | | [[Category: Zhang, J P.]] |
| [[Category: Beta barrel trimer]] | | [[Category: Beta barrel trimer]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:31:10 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:54:17 2008'' |
