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| [[Image:3d26.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_3d26| PDB=3d26 | SCENE= }} | | {{STRUCTURE_3d26| PDB=3d26 | SCENE= }} |
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| '''Norwalk P domain A-trisaccharide complex'''
| | ===Norwalk P domain A-trisaccharide complex=== |
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| ==Overview==
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| Noroviruses are positive sense, single-stranded RNA viruses that cause acute gastroenteritis. They recognize human histo-blood group antigens as receptors in a strain-specific manner. The structures presented here sought to elucidate the structural basis for differences in ligand recognition of noroviruses from different genogroups, the prototypic Norwalk virus (NV, GI-1) and VA387 (GII-4), which recognize the same A-antigen, but differ in the inability of NV to bind to the B-antigen. Two forms of the receptor-binding domain of the norovirus coat protein, the P domain and the P polypeptide, that were previously shown to differ in receptor binding and P particle formation properties were studied. Comparison of the structures of the NV P domain with and without A-trisaccharide and the NV P polypeptide reveal no major ligand-induced changes. The 2.3 A co-crystal structure reveals that the A-trisaccharide binds to the NV P domain through interactions with residues Ser377, Asp327, His329, and Ser380 in a mode distinct from that previously reported for the VA387 P domain-A trisaccharide complex. Mutational analyses confirm the importance of these residues in NV P particle binding to native A-antigen. The alpha-GalNac residue unique to the A-trisaccharide is buried deeply in the NV binding pocket, unlike in the structures of A- and B-trisaccharides bound to VA387 P domain where the alpha-fucose residue forms the most protein contacts. The A-trisaccharide binding mode seen in the NV P domain complex cannot be sterically accommodated in the VA387 P domain.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18385236}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18385236 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18385236}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Norwalk p domain a trisaccaride complex]] | | [[Category: Norwalk p domain a trisaccaride complex]] |
| [[Category: Viral protein]] | | [[Category: Viral protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:50:56 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:10:09 2008'' |
