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| [[Image:1s7k.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1s7k| PDB=1s7k | SCENE= }} | | {{STRUCTURE_1s7k| PDB=1s7k | SCENE= }} |
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| '''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form 2 (apo)'''
| | ===RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form 2 (apo)=== |
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| ==Overview==
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| RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15817456}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15817456 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15817456}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Gnat]] | | [[Category: Gnat]] |
| [[Category: L7/l12]] | | [[Category: L7/l12]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:23:43 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:23:21 2008'' |
