2h7v: Difference between revisions

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[[Image:2h7v.gif|left|200px]]
{{Seed}}
[[Image:2h7v.png|left|200px]]


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{{STRUCTURE_2h7v|  PDB=2h7v  |  SCENE=  }}  
{{STRUCTURE_2h7v|  PDB=2h7v  |  SCENE=  }}  


'''Co-crystal structure of YpkA-Rac1'''
===Co-crystal structure of YpkA-Rac1===




==Overview==
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Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.
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{{ABSTRACT_PUBMED_16959567}}


==About this Structure==
==About this Structure==
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[[Category: Yopo]]
[[Category: Yopo]]
[[Category: Ypka]]
[[Category: Ypka]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:58:24 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:23:42 2008''

Revision as of 08:23, 29 July 2008

File:2h7v.png

Template:STRUCTURE 2h7v

Co-crystal structure of YpkA-Rac1

Template:ABSTRACT PUBMED 16959567

About this Structure

2H7V is a Protein complex structure of sequences from Homo sapiens and Yersinia pseudotuberculosis. Full crystallographic information is available from OCA.

Reference

Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:16959567

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