2jvn: Difference between revisions

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[[Image:2jvn.jpg|left|200px]]
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{{STRUCTURE_2jvn|  PDB=2jvn  |  SCENE=  }}  
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'''Domain C of human PARP-1'''
===Domain C of human PARP-1===




==Overview==
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Poly(ADP-ribose) polymerase-1 (PARP-1) is a multimodular nuclear protein that participates in many fundamental cellular activities. Stimulated by binding to nicked DNA, PARP-1 catalyzes poly(ADP-ribosyl)ation of the acceptor proteins using NAD (+) as a substrate. In this work, NMR methods were used to determine the solution structure of human PARP-1 protein. Domain C was found to contain a zinc-binding motif of three antiparallel beta-strands with four conserved cysteines positioned to coordinate the metal ligand, in addition to a helical region. The zinc-binding motif is structurally reminiscent of the "zinc-ribbon" fold, but with a novel spacing between the conserved cysteines (C X 2C X 12C X 9C). Domain C alone does not appear to bind to DNA. Interestingly, domain C is essential for PARP-1 activity, since a mixture containing nicked DNA and the PARP-1 ABDEF domains has only basal enzymatic activity, while the addition of domain C to the mixture initiated NAD (+) hydrolysis and the formation of poly(ADP-ribose), as detected by an NMR-based assay and autoradiography. The structural model for domain C in solution provides an important framework for further studies aimed at improving our understanding of how the various domains within the complex PARP-1 enzyme play their respective roles in regulating the enzyme activity when cells are under conditions of genotoxic stress.
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==About this Structure==
==About this Structure==
2JVN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVN OCA].  
2JVN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVN OCA].  


==Reference==
==Reference==
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[[Category: Zinc]]
[[Category: Zinc]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 14 11:19:58 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:55:26 2008''

Revision as of 08:55, 29 July 2008

File:2jvn.png

Template:STRUCTURE 2jvn

Domain C of human PARP-1

Template:ABSTRACT PUBMED 18452307

About this Structure

2JVN is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Domain C of Human Poly(ADP-ribose) Polymerase-1 Is Important for Enzyme Activity and Contains a Novel Zinc-Ribbon Motif(,)., Tao Z, Gao P, Hoffman DW, Liu HW, Biochemistry. 2008 May 2;. PMID:18452307

Page seeded by OCA on Tue Jul 29 11:55:26 2008

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