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| {{STRUCTURE_2zg3| PDB=2zg3 | SCENE= }} | | {{STRUCTURE_2zg3| PDB=2zg3 | SCENE= }} |
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| '''Crystal Structure of Two N-terminal Domains of Native Siglec-5 in Complex with 3'-Sialyllactose'''
| | ===Crystal Structure of Two N-terminal Domains of Native Siglec-5 in Complex with 3'-Sialyllactose=== |
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| ==Overview==
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| Sialic acid (Sia) Ig-like binding lectins are important mediators of recognition and signaling events among myeloid cells. To investigate the molecular mechanism underlying sialic acid Ig-like lectin (Siglec) functions, we determined the crystal structure of the two N-terminal extracellular domains of human myeloid cell inhibitory receptor Siglec-5 (CD170) and its complexes with two sialylated carbohydrates. The native structure revealed an unusual conformation of the CC' ligand specificity loop and a unique interdomain disulfide bond. The alpha(2,3)- and alpha(2,6)-sialyllactose complexed structures showed a conserved Sia recognition motif that involves both Arg124 and a portion of the G-strand in the V-set domain forming beta-sheet-like hydrogen bonds with the glycerol side chain of the Sia. Only few protein contacts to the subterminal sugars are observed and mediated by the highly variable GG' linker and CC' loop. These structural observations, in conjunction with surface plasmon resonance binding assays, provide mechanistic insights into linkage-dependent Siglec carbohydrate recognition and suggest that Siglec-5 and other CD33-related Siglec receptors are more promiscuous in sialoglycan recognition than previously understood.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18022638}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18022638 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18022638}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Two-domain structure]] | | [[Category: Two-domain structure]] |
| [[Category: V-set]] | | [[Category: V-set]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:12:20 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:21:48 2008'' |
