2hki: Difference between revisions

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[[Image:2hki.gif|left|200px]]
{{Seed}}
[[Image:2hki.png|left|200px]]


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{{STRUCTURE_2hki|  PDB=2hki  |  SCENE=  }}  
{{STRUCTURE_2hki|  PDB=2hki  |  SCENE=  }}  


'''Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform'''
===Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform===




==Overview==
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Two crystalline forms of GADPH (D-glyceraldehyde-3-phosphate dehydrogenase) from Spinacia oleracea were obtained using sitting-drop vapor diffusion. Despite the very low concentration of GADPH in the solutions, two crystalline forms were obtained, one of which was the previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 A and the other of which belonged to a new space group I4(1)22, with unit-cell parameters a = b = 120.9, c = 154.5 A. Diffraction data were measured from both native and derivatives, yielding structures at a resolution limit of 3.0 A. Differences at the NAD(+)/NADP(+)-binding site seen in these structures compared with the previously reported structure with bound coenzyme suggest that conformational changes associated with pyridine-nucleotide binding may play a role in the regulation of this enzyme.
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{{ABSTRACT_PUBMED_17077485}}


==About this Structure==
==About this Structure==
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[[Category: Apo-form]]
[[Category: Apo-form]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:23:56 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:25:29 2008''

Revision as of 09:25, 29 July 2008

File:2hki.png

Template:STRUCTURE 2hki

Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform

Template:ABSTRACT PUBMED 17077485

About this Structure

2HKI is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.

Reference

Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1087-92. Epub 2006 Oct 25. PMID:17077485

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