3bxd: Difference between revisions

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[[Image:3bxd.jpg|left|200px]]
{{Seed}}
[[Image:3bxd.png|left|200px]]


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{{STRUCTURE_3bxd|  PDB=3bxd  |  SCENE=  }}  
{{STRUCTURE_3bxd|  PDB=3bxd  |  SCENE=  }}  


'''Crystal structure of Mouse Myo-inositol oxygenase (re-refined)'''
===Crystal structure of Mouse Myo-inositol oxygenase (re-refined)===




==Overview==
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Altered inositol metabolism is implicated in a number of diabetic complications. The first committed step in mammalian inositol catabolism is performed by myo-inositol oxygenase (MIOX), which catalyzes a unique four-electron dioxygen-dependent ring cleavage of myo-inositol to d-glucuronate. Here, we present the crystal structure of human MIOX in complex with myo-inosose-1 bound in a terminal mode to the MIOX diiron cluster site. Furthermore, from biochemical and biophysical results from N-terminal deletion mutagenesis we show that the N terminus is important, through coordination of a set of loops covering the active site, in shielding the active site during catalysis. EPR spectroscopy of the unliganded enzyme displays a two-component spectrum that we can relate to an open and a closed active site conformation. Furthermore, based on site-directed mutagenesis in combination with biochemical and biophysical data, we propose a novel role for Lys(127) in governing access to the diiron cluster.
The line below this paragraph, {{ABSTRACT_PUBMED_18364358}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_18364358}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural and Biophysical Characterization of Human myo-Inositol Oxygenase., Thorsell AG, Persson C, Voevodskaya N, Busam RD, Hammarstrom M, Graslund S, Graslund A, Hallberg BM, J Biol Chem. 2008 May 30;283(22):15209-16. Epub 2008 Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18364358 18364358]
Structural and Biophysical Characterization of Human myo-Inositol Oxygenase., Thorsell AG, Persson C, Voevodskaya N, Busam RD, Hammarstrom M, Graslund S, Graslund A, Hallberg BM, J Biol Chem. 2008 May 30;283(22):15209-16. Epub 2008 Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18364358 18364358]
Crystal structure of a substrate complex of myo-inositol oxygenase, a di-iron oxygenase with a key role in inositol metabolism., Brown PM, Caradoc-Davies TT, Dickson JM, Cooper GJ, Loomes KM, Baker EN, Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15032-7. Epub 2006 Sep 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17012379 17012379]
[[Category: Inositol oxygenase]]
[[Category: Inositol oxygenase]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Protein-substrate complex]]
[[Category: Protein-substrate complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:57:45 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:32:34 2008''

Revision as of 09:32, 29 July 2008

File:3bxd.png

Template:STRUCTURE 3bxd

Crystal structure of Mouse Myo-inositol oxygenase (re-refined)

Template:ABSTRACT PUBMED 18364358

About this Structure

3BXD is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase., Thorsell AG, Persson C, Voevodskaya N, Busam RD, Hammarstrom M, Graslund S, Graslund A, Hallberg BM, J Biol Chem. 2008 May 30;283(22):15209-16. Epub 2008 Mar 24. PMID:18364358

Crystal structure of a substrate complex of myo-inositol oxygenase, a di-iron oxygenase with a key role in inositol metabolism., Brown PM, Caradoc-Davies TT, Dickson JM, Cooper GJ, Loomes KM, Baker EN, Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15032-7. Epub 2006 Sep 29. PMID:17012379

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