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| {{STRUCTURE_2pgg| PDB=2pgg | SCENE= }} | | {{STRUCTURE_2pgg| PDB=2pgg | SCENE= }} |
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| '''Crystal Structure of a Birnavirus (IBDV) RNA-dependent RNA Polymerase VP1'''
| | ===Crystal Structure of a Birnavirus (IBDV) RNA-dependent RNA Polymerase VP1=== |
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| ==Overview==
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| Single-subunit polymerases are universally encoded in both cellular organisms and viruses. Their three-dimensional structures have the shape of a right-hand with the active site located in the palm region, which has a topology similar to that of the RNA recognition motif (RRM) found in many RNA-binding proteins. Considering that polymerases have well conserved structures, it was surprising that the RNA-dependent RNA polymerases from birnaviruses, a group of dsRNA viruses, have their catalytic motifs arranged in a permuted order in sequence. Here we report the 2.5 A structure of a birnavirus VP1 in which the polymerase palm subdomain adopts a new active site topology that has not been previously observed in other polymerases. In addition, the polymerase motif C of VP1 has the sequence of -ADN-, a highly unusual feature for RNA-dependent polymerases. Through site-directed mutagenesis, we have shown that changing the VP1 motif C from -ADN- to -GDD- results in a mutant with an increased RNA synthesis activity. Our results indicate that the active site topology of VP1 may represent a newly developed branch in polymerase evolution, and that birnaviruses may have acquired the -ADN- mutation to control their growth rate.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17456597}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17456597 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17456597}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Polymerase]] | | [[Category: Polymerase]] |
| [[Category: Rdrp]] | | [[Category: Rdrp]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:03:47 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:36:24 2008'' |
