2flg: Difference between revisions

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[[Image:2flg.gif|left|200px]]
{{Seed}}
[[Image:2flg.png|left|200px]]


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{{STRUCTURE_2flg|  PDB=2flg  |  SCENE=  }}  
{{STRUCTURE_2flg|  PDB=2flg  |  SCENE=  }}  


'''Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1'''
===Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1===




==Overview==
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The Plasmodium falciparum merozoite surface protein-1 19 kDa fragment (MSP-1(19)) comprises two closely packed EGF-like domains (EGF=epidermal growth factor), each stabilized by three disulfide bonds. The native conformation of this protein is important for eliciting P. falciparum growth inhibitory antibodies. Here we show that the N-terminal EGF domain alone can be chemically synthesized and efficiently refolded to a native-like state, as shown by its solution structure as determined by NMR spectroscopy. In order to study its immunogenicity, the domain was coupled through its N terminus to a phospholipid and incorporated into reconstituted influenza virus-like particles (virosomes). When used to immunize mice, the peptide-loaded virosomes elicited potent humoral immune responses that were shown by Western blots and immunofluorescence assays to cross-react with native MSP-1 on the surfaces of P. falciparum blood stage parasites. This opens the way for a medicinal chemistry-oriented approach to the study and optimization of the antigenicity of the protein as a potential malaria vaccine candidate, whilst exploiting the immunopotentiating properties of influenza virosomes as a delivery vehicle.
The line below this paragraph, {{ABSTRACT_PUBMED_17068840}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 17068840 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17068840}}


==About this Structure==
==About this Structure==
2FLG is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLG OCA].  
2FLG is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLG OCA].  


==Reference==
==Reference==
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[[Category: Surface antigen]]
[[Category: Surface antigen]]
[[Category: Surface protein]]
[[Category: Surface protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:01:51 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:09:21 2008''

Revision as of 10:09, 29 July 2008

File:2flg.png

Template:STRUCTURE 2flg

Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1

Template:ABSTRACT PUBMED 17068840

About this Structure

2FLG is a Single protein structure. Full experimental information is available from OCA.

Reference

Synthesis, solution structure and immune recognition of an epidermal growth factor-like domain from Plasmodium falciparum merozoite surface protein-1., James S, Moehle K, Renard A, Mueller MS, Vogel D, Zurbriggen R, Pluschke G, Robinson JA, Chembiochem. 2006 Dec;7(12):1943-50. PMID:17068840

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