2hob: Difference between revisions

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[[Image:2hob.jpg|left|200px]]
{{Seed}}
[[Image:2hob.png|left|200px]]


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{{STRUCTURE_2hob|  PDB=2hob  |  SCENE=  }}  
{{STRUCTURE_2hob|  PDB=2hob  |  SCENE=  }}  


'''Crystal structure of SARS-CoV main protease with authentic N and C-termini in complex with a Michael acceptor N3'''
===Crystal structure of SARS-CoV main protease with authentic N and C-termini in complex with a Michael acceptor N3===




==Overview==
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The viral proteases have proven to be the most selective and useful for removing the fusion tags in fusion protein expression systems. As a key enzyme in the viral life-cycle, the main protease (M(pro)) is most attractive for drug design targeting the SARS coronavirus (SARS-CoV), the etiological agent responsible for the outbreak of severe acute respiratory syndrome (SARS) in 2003. In this study, SARS-CoV M(pro) was used to specifically remove the GST tag in a new fusion protein expression system. We report a new method to produce wild-type (WT) SARS-CoV M(pro) with authentic N and C termini, and compare the activity of WT protease with those of three different types of SARS-CoV M(pro) with additional residues at the N or C terminus. Our results show that additional residues at the N terminus, but not at the C terminus, of M(pro) are detrimental to enzyme activity. To explain this, the crystal structures of WT SARS-CoV M(pro) and its complex with a Michael acceptor inhibitor were determined to 1.6 Angstroms and 1.95 Angstroms resolution respectively. These crystal structures reveal that the first residue of this protease is important for sustaining the substrate-binding pocket and inhibitor binding. This study suggests that SARS-CoV M(pro) could serve as a new tag-cleavage endopeptidase for protein overproduction, and the WT SARS-CoV M(pro) is more appropriate for mechanistic characterization and inhibitor design.
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{{ABSTRACT_PUBMED_17189639}}


==About this Structure==
==About this Structure==
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[[Category: Michael acceptor n3]]
[[Category: Michael acceptor n3]]
[[Category: Sars-cov]]
[[Category: Sars-cov]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:30:55 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:32:52 2008''

Revision as of 10:32, 29 July 2008

File:2hob.png

Template:STRUCTURE 2hob

Crystal structure of SARS-CoV main protease with authentic N and C-termini in complex with a Michael acceptor N3

Template:ABSTRACT PUBMED 17189639

About this Structure

2HOB is a Single protein structure of sequence from Sars coronavirus. Full crystallographic information is available from OCA.

Reference

Production of authentic SARS-CoV M(pro) with enhanced activity: application as a novel tag-cleavage endopeptidase for protein overproduction., Xue X, Yang H, Shen W, Zhao Q, Li J, Yang K, Chen C, Jin Y, Bartlam M, Rao Z, J Mol Biol. 2007 Feb 23;366(3):965-75. Epub 2006 Dec 1. PMID:17189639

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