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| [[Image:1okf.gif|left|200px]] | | {{Seed}} |
| | [[Image:1okf.png|left|200px]] |
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| {{STRUCTURE_1okf| PDB=1okf | SCENE= }} | | {{STRUCTURE_1okf| PDB=1okf | SCENE= }} |
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| '''NMR STRUCTURE OF AN ALPHA-L-LNA:RNA HYBRID'''
| | ===NMR STRUCTURE OF AN ALPHA-L-LNA:RNA HYBRID=== |
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| ==Overview==
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| Alpha-L-LNA (alpha-L-ribo configured locked nucleic acid) is a nucleotide analogue that raises the thermostability of nucleic acid duplexes by up to approximately 4 degrees C per inclusion. We have determined the NMR structure of a nonamer alpha-L-LNA:RNA hybrid with three alpha-L-LNA modifications. The geometry of this hybrid is intermediate between A- and B-type, all nucleobases partake in Watson-Crick base pairing and base stacking, and the global structure is very similar to that of the corresponding unmodified hybrid. The sugar-phosphate backbone is rearranged in the vicinity of the modified nucleotides. As a consequence, the phosphate groups following the modified nucleotides are rotated into the minor groove. It is interesting that the alpha-L-LNA:RNA hybrid, which has an elevation in melting temperature of 17 degrees C relative to the corresponding DNA:RNA hybrid, retains the global structure of this hybrid. To our knowledge, this is the first example of such a substantial increase in melting temperature of a nucleic acid analogue that does not act as an N-type (RNA) mimic. alpha-L-LNA:RNA hybrids are recognised by RNase H with subsequent cleavage of the RNA strand, albeit with slow rates. We attempt to rationalise this impaired enzyme activity from the rearrangement of the sugar-phosphate backbone of the alpha-L-LNA:RNA hybrid.
| | The line below this paragraph, {{ABSTRACT_PUBMED_14530434}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 14530434 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_14530434}} |
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| ==About this Structure== | | ==About this Structure== |
| 1OKF is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKF OCA]. | | 1OKF is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKF OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Rna]] | | [[Category: Rna]] |
| [[Category: Rnase h]] | | [[Category: Rnase h]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:57:59 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:38:47 2008'' |
