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| {{STRUCTURE_2bw4| PDB=2bw4 | SCENE= }} | | {{STRUCTURE_2bw4| PDB=2bw4 | SCENE= }} |
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| '''ATOMIC RESOLUTION STRUCTURE OF RESTING STATE OF THE ACHROMOBACTER CYCLOCLASTES CU NITRITE REDUCTASE'''
| | ===ATOMIC RESOLUTION STRUCTURE OF RESTING STATE OF THE ACHROMOBACTER CYCLOCLASTES CU NITRITE REDUCTASE=== |
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| ==Overview==
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| Copper-containing nitrite reductases catalyze the reduction of nitrite to nitric oxide (NO), a key step in denitrification that results in the loss of terrestrial nitrogen to the atmosphere. They are found in a wide variety of denitrifying bacteria and fungi of different physiology from a range of soil and aquatic ecosystems. Structural analysis of potential intermediates in the catalytic cycle is an important goal in understanding enzyme mechanism. Using "crystal harvesting" and substrate-soaking techniques, we have determined atomic resolution structures of four forms of the green Cu-nitrite reductase, from the soil bacterium Achromobacter cycloclastes. These structures are the resting state of the enzyme at 0.9 A, two species exhibiting different conformations of nitrite bound at the catalytic type 2 Cu, one of which is stable and also has NO present, at 1.10 A and 1.15 A, and a stable form with the product NO bound side-on to the catalytic type 2 Cu, at 1.12 A resolution. These structures provide incisive insights into the initial binding of substrate, its repositioning before catalysis, bond breakage (O-NO), and the formation of a stable NO adduct.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16093314}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16093314 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16093314}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Denitrification,catalysis,enzyme mechanism,oxidoreductase]] | | [[Category: Denitrification,catalysis,enzyme mechanism,oxidoreductase]] |
| [[Category: Nitrate assimilation]] | | [[Category: Nitrate assimilation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:53:12 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:39:38 2008'' |
