1rxz: Difference between revisions

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[[Image:1rxz.gif|left|200px]]
{{Seed}}
[[Image:1rxz.png|left|200px]]


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{{STRUCTURE_1rxz|  PDB=1rxz  |  SCENE=  }}  
{{STRUCTURE_1rxz|  PDB=1rxz  |  SCENE=  }}  


'''C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA'''
===C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA===




==Overview==
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Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
The line below this paragraph, {{ABSTRACT_PUBMED_14718165}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 14718165 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14718165}}


==About this Structure==
==About this Structure==
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[[Category: Dna replication]]
[[Category: Dna replication]]
[[Category: Interdomain connecting loop]]
[[Category: Interdomain connecting loop]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:02:42 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:14:01 2008''

Revision as of 11:14, 29 July 2008

File:1rxz.png

Template:STRUCTURE 1rxz

C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA

Template:ABSTRACT PUBMED 14718165

About this Structure

1RXZ is a Protein complex structure of sequences from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:14718165

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