2a63: Difference between revisions

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[[Image:2a63.gif|left|200px]]
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[[Image:2a63.png|left|200px]]


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{{STRUCTURE_2a63|  PDB=2a63  |  SCENE=  }}  
{{STRUCTURE_2a63|  PDB=2a63  |  SCENE=  }}  


'''Solution structure of a stably monomeric mutant of lambda Cro produced by substitutions in the ball-and-socket interface'''
===Solution structure of a stably monomeric mutant of lambda Cro produced by substitutions in the ball-and-socket interface===




==Overview==
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The homodimeric lambda Cro protein has a "ball-and-socket" interface that includes insertion of an aromatic side chain, Phe 58, from each subunit into a cavity in the hydrophobic core of the other subunit. This overlap between the subunit core and dimer interface hypothetically explains the strong dimerization and weak monomer stability of lambda Cro in comparison to homologues. According to a model developed here and in a previous study [LeFevre, K. R., and Cordes, M. H. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 2345-2350], the socket cavity evolved in part by replacement of a buried tryptophan in an ancestral stable monomer with a smaller side chain (Ala 33 in lambda Cro). The resulting core defect was in effect repaired by insertion of a different side chain (Phe 58) from a second subunit, generating the ball and socket. Consistent with such an evolutionary trade between intrasubunit and intersubunit interactions, we showed in the previous study that restoration of the ancestral Trp 33 in lambda Cro stabilized the monomer and reduced the extent of dimerization. Here, we report the solution structure of a stable lambda Cro monomer containing the Ala33Trp mutation, which confirms that the restored tryptophan fulfills its ancestral role as a core side chain, filling part of the socket cavity occupied by Phe 58 in the wild-type dimer. The structure also reveals, however, that the cavity is not completely filled by Trp 33, suggesting that its formation could have involved multiple mutations that reduced side chain volume. We offer suggestive evidence of a role of mutations at a second position.
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{{ABSTRACT_PUBMED_16700549}}


==About this Structure==
==About this Structure==
2A63 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A63 OCA].  
2A63 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A63 OCA].  


==Reference==
==Reference==
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[[Category: Helix-turn-helix]]
[[Category: Helix-turn-helix]]
[[Category: Monomer]]
[[Category: Monomer]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:39:15 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:26:51 2008''

Revision as of 11:26, 29 July 2008

File:2a63.png

Template:STRUCTURE 2a63

Solution structure of a stably monomeric mutant of lambda Cro produced by substitutions in the ball-and-socket interface

Template:ABSTRACT PUBMED 16700549

About this Structure

2A63 is a Single protein structure of sequence from Enterobacteria phage lambda. Full experimental information is available from OCA.

Reference

A trade between similar but nonequivalent intrasubunit and intersubunit contacts in Cro dimer evolution., Newlove T, Atkinson KR, Van Dorn LO, Cordes MH, Biochemistry. 2006 May 23;45(20):6379-91. PMID:16700549

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