2olc: Difference between revisions

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[[Image:2olc.jpg|left|200px]]
{{Seed}}
[[Image:2olc.png|left|200px]]


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{{STRUCTURE_2olc|  PDB=2olc  |  SCENE=  }}  
{{STRUCTURE_2olc|  PDB=2olc  |  SCENE=  }}  


'''Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho'''
===Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho===




==Overview==
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Trivalent holmium ions were shown to isomorphously replace magnesium ions to form an ADP-2Ho complex in the nucleotide-binding domain of Bacillus subtilis 5-methylthioribose (MTR) kinase. This nucleotide-holmium complex provided sufficient phasing power to allow SAD and SIRAS phasing of this previously unknown structure using the L(III) absorption edge of holmium. The structure of ADP-2Ho reveals that the two Ho ions are approximately 4 A apart and are likely to share their ligands: the phosphoryl O atoms of ADP and a water molecule. The structure determination of MTR kinase using data collected using Cu Kalpha X-radiation was also attempted. Although the heavy-atom substructure determination was successful, interpretation of the map was more challenging. The isomorphous substitution of holmium for magnesium in the MTR kinase-nucleotide complex suggests that this could be a useful phasing tool for other metal-dependent nucleotide-containing proteins.
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{{ABSTRACT_PUBMED_17372354}}


==About this Structure==
==About this Structure==
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[[Category: Smith, G D.]]
[[Category: Smith, G D.]]
[[Category: Kinase adp-2ho complex]]
[[Category: Kinase adp-2ho complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 11:09:06 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:47:09 2008''

Revision as of 11:47, 29 July 2008

File:2olc.png

Template:STRUCTURE 2olc

Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho

Template:ABSTRACT PUBMED 17372354

About this Structure

2OLC is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

ADP-2Ho as a phasing tool for nucleotide-containing proteins., Ku SY, Smith GD, Howell PL, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):493-9. Epub 2007, Mar 16. PMID:17372354

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