1wky: Difference between revisions

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~~'''~~Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module~~'''~~

===Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module===

~~==Overview==~~

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~~An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced.~~ The ~~deduced amino-acid sequence of~~ the ~~enzyme suggested that~~ the ~~enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method~~ at ~~277 K. X-ray diffraction data were collected to 1~~.~~65 A~~. ~~The crystal belongs to space group P2(1)2(1)2(1~~), ~~with unit-cell parameters a = 70~~.~~7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(~~-~~1) and a solvent content of 45.6%. Molecular replacement for initial phasing was carried out using the three~~-dimensional structure of a mannanase from Thermomonospora fusca as a search model, which corresponds to the catalytic domain of the alkaline mannanase. It gave sufficient phases to build the unknown domain.

The line below this paragraph, {{ABSTRACT_PUBMED_15272186}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15272186 is the PubMed ID number.

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==About this Structure==

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[[Category: Cbm]]

[[Category: Tim barrel]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 13~~:49:17 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:49:50 2008''

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-[[Image:1wky.gif|left|200px]]
+{{Seed}}
+[[Image:1wky.png|left|200px]]
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 {{STRUCTURE_1wky|  PDB=1wky  |  SCENE=  }}
-'''Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module'''
+===Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module===
-==Overview==
+<!--
-An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence of the enzyme suggested that the enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method at 277 K. X-ray diffraction data were collected to 1.65 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(-1) and a solvent content of 45.6%. Molecular replacement for initial phasing was carried out using the three-dimensional structure of a mannanase from Thermomonospora fusca as a search model, which corresponds to the catalytic domain of the alkaline mannanase. It gave sufficient phases to build the unknown domain.
+The line below this paragraph, {{ABSTRACT_PUBMED_15272186}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15272186 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15272186}}
 ==About this Structure==
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 [[Category: Cbm]]
 [[Category: Tim barrel]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:49:17 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:49:50 2008''