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[[Image:1zda.jpg|left|200px]]
{{Seed}}
[[Image:1zda.png|left|200px]]


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{{STRUCTURE_1zda|  PDB=1zda  |  SCENE=  }}  
{{STRUCTURE_1zda|  PDB=1zda  |  SCENE=  }}  


'''PHAGE-SELECTED MINI PROTEIN A DOMAIN, Z38, NMR, 24 STRUCTURES'''
===PHAGE-SELECTED MINI PROTEIN A DOMAIN, Z38, NMR, 24 STRUCTURES===




==Overview==
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The affinity between molecules depends both on the nature and presentation of the contacts. Here, we observe coupling of functional and structural elements when a protein binding domain is evolved to a smaller functional mimic. Previously, a 38-residue form of the 59-residue B-domain of protein A, termed Z38, was selected by phage display. Z38 contains 13 mutations and binds IgG only 10-fold weaker than the native B-domain. We present the solution structure of Z38 and show that it adopts a tertiary structure remarkably similar to that observed for the first two helices of B-domain in the B-domain/Fc complex [Deisenhofer, J. (1981) Biochemistry 20, 2361-2370], although it is significantly less stable. Based on this structure, we have improved on Z38 by designing a 34-residue disulfide-bonded variant (Z34C) that has dramatically enhanced stability and binds IgG with 9-fold higher affinity. The improved stability of Z34C led to NMR spectra with much greater chemical shift dispersion, resulting in a more precisely determined structure. Z34C, like Z38, has a structure virtually identical to the equivalent region from native protein A domains. The well-defined hydrophobic core of Z34C reveals key structural features that have evolved in this small, functional domain. Thus, the stabilized two-helix peptide, about half the size and having one-third of the remaining residues altered, accurately mimics both the structure and function of the native domain.
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{{ABSTRACT_PUBMED_9294166}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDA OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDA OCA].  


==Reference==
==Reference==
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[[Category: Igg binding domain]]
[[Category: Igg binding domain]]
[[Category: Protein a mimic]]
[[Category: Protein a mimic]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:29:19 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:19:16 2008''

Revision as of 12:19, 29 July 2008

File:1zda.png

Template:STRUCTURE 1zda

PHAGE-SELECTED MINI PROTEIN A DOMAIN, Z38, NMR, 24 STRUCTURES

Template:ABSTRACT PUBMED 9294166

About this Structure

Full experimental information is available from OCA.

Reference

Structural mimicry of a native protein by a minimized binding domain., Starovasnik MA, Braisted AC, Wells JA, Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10080-5. PMID:9294166

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