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| {{STRUCTURE_1ow1| PDB=1ow1 | SCENE= }} | | {{STRUCTURE_1ow1| PDB=1ow1 | SCENE= }} |
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| '''Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.'''
| | ===Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.=== |
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| ==Overview==
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| Spen proteins regulate the expression of key transcriptional effectors in diverse signaling pathways. They are large proteins characterized by N-terminal RNA-binding motifs and a highly conserved C-terminal SPOC domain. The specific biological role of the SPOC domain (Spen paralog and ortholog C-terminal domain), and hence, the common function of Spen proteins, has been unclear to date. The Spen protein, SHARP (SMRT/HDAC1-associated repressor protein), was identified as a component of transcriptional repression complexes in both nuclear receptor and Notch/RBP-Jkappa signaling pathways. We have determined the 1.8 A crystal structure of the SPOC domain from SHARP. This structure shows that essentially all of the conserved surface residues map to a positively charged patch. Structure-based mutational analysis indicates that this conserved region is responsible for the interaction between SHARP and the universal transcriptional corepressor SMRT/NCoR (silencing mediator for retinoid and thyroid receptors/nuclear receptor corepressor. We demonstrate that this interaction involves a highly conserved acidic motif at the C terminus of SMRT/NCoR. These findings suggest that the conserved function of the SPOC domain is to mediate interaction with SMRT/NCoR corepressors, and that Spen proteins play an essential role in the repression complex.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12897056}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12897056 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12897056}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Beta-alpha-barrel]] | | [[Category: Beta-alpha-barrel]] |
| [[Category: Spoc domain]] | | [[Category: Spoc domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:20:39 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:20:47 2008'' |
