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| {{STRUCTURE_1tu4| PDB=1tu4 | SCENE= }} | | {{STRUCTURE_1tu4| PDB=1tu4 | SCENE= }} |
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| '''Crystal Structure of Rab5-GDP Complex'''
| | ===Crystal Structure of Rab5-GDP Complex=== |
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| ==Overview==
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| Rab5 is a small GTPase that regulates early endosome fusion. We present here the crystal structure of the Rab5 GTPase domain in complex with a GTP analog and the C-terminal domain of effector Rabaptin5. The proteins form a dyad-symmetric Rab5-Rabaptin5(2)-Rab5 ternary complex with a parallel coiled-coil Rabaptin5 homodimer in the middle. Two Rab5 molecules bind independently to the Rabaptin5 dimer using their switch and interswitch regions. The binding does not involve the Rab complementarity-determining regions. We also present the crystal structures of two distinct forms of GDP-Rab5 complexes, both of which are incompatible with Rabaptin5 binding. One has a dislocated and disordered switch I but a virtually intact switch II, whereas the other has its beta-sheet and both switch regions reorganized. Biochemical and functional analyses show that the crystallographically observed Rab5-Rabaptin5 complex also exists in solution, and disruption of this complex by mutation abrogates endosome fusion.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15378032}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15378032 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15378032}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Gtpase]] | | [[Category: Gtpase]] |
| [[Category: Rab5]] | | [[Category: Rab5]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:22:00 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:37:29 2008'' |
