2abw: Difference between revisions

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[[Image:2abw.gif|left|200px]]
{{Seed}}
[[Image:2abw.png|left|200px]]


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{{STRUCTURE_2abw|  PDB=2abw  |  SCENE=  }}  
{{STRUCTURE_2abw|  PDB=2abw  |  SCENE=  }}  


'''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)'''
===Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)===




==Overview==
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Vitamin B6 is one of nature's most versatile cofactors. Most organisms synthesize vitamin B6 via a recently discovered pathway employing the proteins Pdx1 and Pdx2. Here we present an in-depth characterization of the respective orthologs from the malaria parasite, Plasmodium falciparum. Expression profiling of Pdx1 and -2 shows that blood-stage parasites indeed possess a functional vitamin B6 de novo biosynthesis. Recombinant Pdx1 and Pdx2 form a complex that functions as a glutamine amidotransferase with Pdx2 as the glutaminase and Pdx1 as pyridoxal-5 '-phosphate synthase domain. Complex formation is required for catalytic activity of either domain. Pdx1 forms a chimeric bi-enzyme with the bacterial YaaE, a Pdx2 ortholog, both in vivo and in vitro, although this chimera does not attain full catalytic activity, emphasizing that species-specific structural features govern the interaction between the protein partners of the PLP synthase complexes in different organisms. To gain insight into the activation mechanism of the parasite bi-enzyme complex, the three-dimensional structure of Pdx2 was determined at 1.62 A. The obstruction of the oxyanion hole indicates that Pdx2 is in a resting state and that activation occurs upon Pdx1-Pdx2 complex formation.
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{{ABSTRACT_PUBMED_16339145}}


==About this Structure==
==About this Structure==
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[[Category: Plp-synthase]]
[[Category: Plp-synthase]]
[[Category: Vitamin b6]]
[[Category: Vitamin b6]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:51:38 2008''
 
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Revision as of 13:03, 29 July 2008

File:2abw.png

Template:STRUCTURE 2abw

Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)

Template:ABSTRACT PUBMED 16339145

About this Structure

2ABW is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.

Reference

Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:16339145

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