2fq6: Difference between revisions

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[[Image:2fq6.jpg|left|200px]]
{{Seed}}
[[Image:2fq6.png|left|200px]]


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{{STRUCTURE_2fq6|  PDB=2fq6  |  SCENE=  }}  
{{STRUCTURE_2fq6|  PDB=2fq6  |  SCENE=  }}  


'''Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-trifluoromethyl-benzamide'''
===Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-trifluoromethyl-benzamide===




==Overview==
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The biosynthesis of methionine is an attractive antibiotic target given its importance in protein and DNA metabolism and its absence in mammals. We have performed a high-throughput screen of the methionine biosynthesis enzyme cystathionine beta-lyase (CBL) against a library of 50 000 small molecules and have identified several compounds that inhibit CBL enzyme activity in vitro. These hit molecules were of two classes: those that blocked CBL activity with mixed steady-state inhibition and those that covalently interacted with the enzyme at the active site pyridoxal phosphate cofactor with slow-binding inhibition kinetics. We determined the crystal structure of one of the slow-binding inhibitors in complex with CBL and used this structure as a guide in the synthesis of a small, focused library of analogues, some of which had improved enzyme inhibition properties. These studies provide the first lead molecules for antimicrobial agents that target cystathionine beta-lyase in methionine biosynthesis.
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{{ABSTRACT_PUBMED_17300162}}


==About this Structure==
==About this Structure==
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[[Category: Plp cofactor covalently bound to p3f inhibitor]]
[[Category: Plp cofactor covalently bound to p3f inhibitor]]
[[Category: Protein-inhibitor complex]]
[[Category: Protein-inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:16:16 2008''

Revision as of 13:16, 29 July 2008

File:2fq6.png

Template:STRUCTURE 2fq6

Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-trifluoromethyl-benzamide

Template:ABSTRACT PUBMED 17300162

About this Structure

2FQ6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Inhibitors of bacterial cystathionine beta-lyase: leads for new antimicrobial agents and probes of enzyme structure and function., Ejim LJ, Blanchard JE, Koteva KP, Sumerfield R, Elowe NH, Chechetto JD, Brown ED, Junop MS, Wright GD, J Med Chem. 2007 Feb 22;50(4):755-64. PMID:17300162

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